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PMID: 8467797 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Functional conservation of vertebrate seven-up related genes in neurogenesis and eye development.

The EMBO journal ·Vol. 12 ·No. 4 ·1993-04-00 ·Pages 1403-14

Fjose A, Nornes S, Weber U, Mlodzik M

Abstract

Several members of the steroid receptor superfamily, including the transcription factor COUP, are closely related to the Drosophila gene seven-up (svp) which is required for the development of the embryonic central nervous system (CNS) and specific photoreceptor cells of the eye. We have identified and characterized two zebrafish (Brachydanio rerio) members of this subfamily of orphan nuclear receptors. While one of them (svp[44]) is the zebrafish cognate of COUP, the second (svp[46]) seems to be a novel member of the COUP/svp group. The proteins encoded by both genes contain highly conserved DNA-binding and putative ligand-binding domains, indicating close similarities in target sequence recognition and ligand binding. Analysis of the spatial distribution of their transcripts in whole-mount embryos revealed that the CNS is a major site of expression for both genes. At early embryonic stages, both genes are expressed in domains corresponding to specific rhombomere primordia in the hindbrain. This suggests an involvement in hindbrain segmentation and/or rhombomere specification. Moreover, transcripts derived from both genes are detected within distinct areas of the eye rudiments, suggesting roles in eye patterning and/or cell differentiation. In the case of the svp[44] gene, expression is also observed within specific parts of the midbrain, diencephalon and telencephalon. These results represent the first evidence that at least some of the nervous system and eye-specific functions of Drosophila svp are conserved in vertebrates.

MeSH Terms
Amino Acid Sequence Animals Base Sequence COUP Transcription Factor I Cloning, Molecular DNA/genetics DNA-Binding Proteins/genetics Eye/embryology Gene Expression In Situ Hybridization Molecular Sequence Data Morphogenesis Nervous System/embryology Nuclear Proteins/genetics Phylogeny RNA, Messenger/genetics Sequence Alignment Transcription Factors/genetics Zebrafish/embryology,genetics Zebrafish Proteins
Chemicals
COUP Transcription Factor I DNA-Binding Proteins Nuclear Proteins RNA, Messenger Transcription Factors Zebrafish Proteins nr2f1a protein, zebrafish nr2f5 protein, zebrafish DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Fjose A
Department of Molecular Genetics, University of Tromsø, Norway.
Nornes S
Weber U
Mlodzik M
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-04-00
Pages
1403-14
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413351
Subset
IM
Databases
GENBANK
X70299, X70300
External Links
PubMed source PMC Full Text View journal details
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