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PMID: 8464924 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Molecular cloning and expression of cDNA for mammalian translation initiation factor 5.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 7 ·1993-04-01 ·Pages 3058-62

Das K, Chevesich J, Maitra U

Abstract

Eukaryotic translation initiation factor 5 (eIF-5) catalyzes the hydrolysis of GTP bound to the 40S ribosomal initiation complex (40S.AUG.Met-tRNAf-eIF-2.GTP) with the subsequent joining of a 60S ribosomal subunit resulting in the formation of a functional 80S initiation complex. A rat cDNA that encodes eIF-5 has been isolated and expressed in Escherichia coli to yield a catalytically active eIF-5 protein. The 3.55-kb cDNA encodes a protein of 429 amino acids (calculated M(r) 48,926) with properties that are similar to eIF-5 isolated from rabbit reticulocyte lysates. The deduced amino acid sequence of eIF-5 contains sequence motifs characteristic of proteins of the GTPase superfamily.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular/methods DNA/genetics Escherichia coli/genetics Eukaryotic Initiation Factor-5 GTP Phosphohydrolases/genetics Guanosine Triphosphate/metabolism Liver/physiology Macromolecular Substances Molecular Sequence Data Multigene Family Oligodeoxyribonucleotides Peptide Initiation Factors/genetics,isolation & purification,metabolism Protein Biosynthesis RNA, Transfer, Amino Acyl/metabolism RNA, Transfer, Met Rabbits Restriction Mapping Reticulocytes/metabolism Ribosomes/metabolism Sequence Homology, Amino Acid
Chemicals
Eukaryotic Initiation Factor-5 Macromolecular Substances Oligodeoxyribonucleotides Peptide Initiation Factors RNA, Transfer, Amino Acyl RNA, Transfer, Met tRNA, formylmethionine- Guanosine Triphosphate DNA GTP Phosphohydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Das K
Department of Developmental Biology and Cancer, Albert Einstein College of Medicine, Bronx, NY 10461.
Chevesich J
Maitra U
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.7.3058
Published
1993-04-01
Pages
3058-62
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC46236
Subset
IM
Grants
NIGMS NIH HHS · GM15399 · United States
NCI NIH HHS · P30CA13330 · United States
Databases
GENBANK
L11651
External Links
PubMed source DOI: 10.1073/pnas.90.7.3058 PMC Full Text View journal details
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