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PMID: 8459805 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Molecular cloning and characterization of the human E-cadherin cDNA.

Molecular biology reports ·Vol. 17 ·No. 2 ·1993-02-00 ·Pages 123-8

Bussemakers MJ, van Bokhoven A, Mees SG, Kemler R, Schalken JA

Abstract

E-cadherin is a Ca(2+)-dependent cell adhesion molecule involved in cell-cell interaction. In its normal physiological function it plays an important role in embryonic development and tissue morphogenesis. Recent studies have shown that in cancer development E-cadherin can act as a suppressor of invasion. Indeed, in several kinds of carcinomas allelic loss of the E-cadherin/Uvomorulin locus and decreased E-cadherin expression have been described. The importance of E-cadherin in human cancer development may be substantiated by molecular analysis of the E-cadherin transcript. Therefore, we isolated and characterized the human E-cadherin cDNA. Comparison of the nucleotide and deduced amino acid sequences revealed that the human E-cadherin is highly homologous to the mouse E-cadherin (uvomorulin) and to other members of the cadherin family.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Cadherins/genetics Cloning, Molecular DNA/genetics Humans Mice Molecular Sequence Data Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid

Chemicals

Cadherins DNA

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Bussemakers M J

Urology Research Laboratory, University Hospital Nijmegen, The Netherlands.

van Bokhoven A

Mees S G

Kemler R

Schalken J A

References (25)

25 references, click to expand

Mutation and cancer: statistical study of retinoblastoma.
Proc Natl Acad Sci U S A. 1971 Apr;68(4):820-3 PMID: 5279523
Genetic manipulation of E-cadherin expression by epithelial tumor cells reveals an invasion suppressor role.
Cell. 1991 Jul 12;66(1):107-19 PMID: 2070412
Cell adhesion molecules: implications for a molecular histology.
Annu Rev Biochem. 1991;60:155-90 PMID: 1883195
The structure of cell adhesion molecule uvomorulin. Insights into the molecular mechanism of Ca2+-dependent cell adhesion.
EMBO J. 1987 Dec 1;6(12):3647-53 PMID: 3501370
Morphoregulatory molecules.
Biochemistry. 1988 May 17;27(10):3533-43 PMID: 3044445
E-cadherin expression in squamous cell carcinomas of head and neck: inverse correlation with tumor dedifferentiation and lymph node metastasis.
Cancer Res. 1991 Dec 1;51(23 Pt 1):6328-37 PMID: 1933895
Allelotype of breast cancer: cumulative allele losses promote tumor progression in primary breast cancer.
Cancer Res. 1990 Nov 15;50(22):7184-9 PMID: 1977515
Isolation of placental cadherin cDNA: identification of a novel gene family of cell-cell adhesion molecules.
EMBO J. 1987 Dec 1;6(12):3655-61 PMID: 3428270
Cadherin intercellular adhesion molecule in hepatocellular carcinomas: loss of E-cadherin expression in an undifferentiated carcinoma.
Cancer Lett. 1991 May 1;57(2):131-5 PMID: 1851053
Sequence analysis of a cDNA clone encoding the liver cell adhesion molecule, L-CAM.
Proc Natl Acad Sci U S A. 1987 May;84(9):2808-12 PMID: 3472238
Deletion mapping of chromosomes 8, 10, and 16 in human prostatic carcinoma.
Genes Chromosomes Cancer. 1991 May;3(3):215-20 PMID: 1868037
Decreased expression of E-cadherin in the progression of rat prostatic cancer.
Cancer Res. 1992 May 15;52(10):2916-22 PMID: 1581909
A comprehensive set of sequence analysis programs for the VAX.
Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387-95 PMID: 6546423
Correct proteolytic cleavage is required for the cell adhesive function of uvomorulin.
J Cell Biol. 1990 Oct;111(4):1645-50 PMID: 2211831
Allelic loss of chromosomes 16q and 10q in human prostate cancer.
Proc Natl Acad Sci U S A. 1990 Nov;87(22):8751-5 PMID: 1978938
Unstable expression of E-cadherin adhesion molecules in metastatic ovarian tumor cells.
Jpn J Cancer Res. 1989 May;80(5):459-63 PMID: 2502522
N-cadherin gene maps to human chromosome 18 and is not linked to the E-cadherin gene.
J Neurochem. 1990 Sep;55(3):805-12 PMID: 2384753
Loss of heterozygosity on 6q, 16q, and 17p in human central nervous system primitive neuroectodermal tumors.
Cancer Res. 1991 Jan 15;51(2):639-43 PMID: 1670763
Allele loss on chromosome 16 associated with progression of human hepatocellular carcinoma.
Proc Natl Acad Sci U S A. 1990 Sep;87(17):6791-4 PMID: 2168560
Characterization and chromosomal localization of the gene encoding the human cell adhesion molecule uvomorulin.
Differentiation. 1988 Jun;38(1):67-71 PMID: 3263290
Uvomorulin-catenin complex formation is regulated by a specific domain in the cytoplasmic region of the cell adhesion molecule.
Proc Natl Acad Sci U S A. 1990 Jun;87(11):4246-50 PMID: 2349235
Dissecting tumor cell invasion: epithelial cells acquire invasive properties after the loss of uvomorulin-mediated cell-cell adhesion.
J Cell Biol. 1989 Jun;108(6):2435-47 PMID: 2661563
Cloning in single-stranded bacteriophage as an aid to rapid DNA sequencing.
J Mol Biol. 1980 Oct 25;143(2):161-78 PMID: 6260957
E-cadherin-mediated cell-cell adhesion prevents invasiveness of human carcinoma cells.
J Cell Biol. 1991 Apr;113(1):173-85 PMID: 2007622
Cell binding function of E-cadherin is regulated by the cytoplasmic domain.
EMBO J. 1988 Dec 1;7(12):3679-84 PMID: 3061804

Article Info

Journal

Molecular biology reports

Abbr.

Mol Biol Rep

ISSN

0301-4851

DOI

10.1007/BF00996219

Published

1993-02-00

Pages

123-8

Language

English

Region

Netherlands

NLM ID

0403234

Subset

Databases

GENBANK

Z13009

External Links

PubMed source DOI: 10.1007/BF00996219 View journal details

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Molecular cloning and characterization of the human E-cadherin cDNA.

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