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PMID: 8441624 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Atomic force microscopy of oriented linear DNA molecules labeled with 5nm gold spheres.

Nucleic acids research ·Vol. 21 ·No. 1 ·1993-01-11 ·Pages 99-103

Shaiu WL, Larson DD, Vesenka J, Henderson E

Abstract

The atomic force microscope (AFM;1) can image DNA and RNA in air and under solutions at resolution comparable to that obtained by electron microscopy (EM) (2-7). We have developed a method for depositing and imaging linear DNA molecules to which 5nm gold spheres have been attached. The gold spheres facilitate orientation of the DNA molecules on the mica surface to which they are absorbed and are potentially useful as internal height standards and as high resolution gene or sequence specific tags. We show that by modulating their adhesion to the mica surface, the gold spheres can be moved with some degree of control with the scanning tip.

MeSH Terms
DNA/ultrastructure Gold Image Processing, Computer-Assisted Microscopy/methods Plasmids
Chemicals
Gold DNA
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Shaiu W L
Department of Zoology and Genetics, Iowa State University, Ames 50011.
Larson D D
Vesenka J
Henderson E
References (12)
12 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.1.99
Published
1993-01-11
Pages
99-103
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309070
Subset
IM
Grants
NIGMS NIH HHS · GM41023 · United States
NIGMS NIH HHS · GM41899 · United States
External Links
PubMed source DOI: 10.1093/nar/21.1.99 PMC Full Text View journal details
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