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PMID: 8441623 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Nucleotide sequence of 5' portion of srfA that contains the region required for competence establishment in Bacillus subtilus.

Nucleic acids research ·Vol. 21 ·No. 1 ·1993-01-11 ·Pages 93-7

Fuma S, Fujishima Y, Corbell N, D'Souza C, Nakano MM, Zuber P, Yamane K

Abstract

The nucleotide sequence of the 20,535 base pairs of the 5' end of the srfA operon, containing the region required for competence development, was determined. This included the srfA promoter region, the first open reading frame, srfAA, encoding surfactin synthetase I and part of the second open reading frame, srfAB, encoding surfactin synthetase II. Three amino acid-activating domains characteristic of those found in peptide synthetases could be discerned in both srfAA (activating Glu, Leu and D-Leu) and srfAB (activating Val, Asp, and D-Leu). The presence of a conserved spacer motif in the amino-terminal end of srfAA suggests that the srfAA product may not initiate surfactin synthesis. The portion of srfA that contains the region required for competence is composed of srfAA and the first amino acid-activating domain of srfAB.

Related Genes
srfA
MeSH Terms
Amino Acid Sequence Bacillus subtilis/enzymology,genetics Bacterial Proteins/genetics Base Sequence DNA, Bacterial/isolation & purification Genes, Bacterial Lipopeptides Molecular Sequence Data Peptide Synthases/genetics Peptides, Cyclic Restriction Mapping Sequence Alignment
Chemicals
Bacterial Proteins DNA, Bacterial Lipopeptides Peptides, Cyclic surfactin peptide Peptide Synthases surfactin synthetase
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Fuma S
Institute of Biological Sciences, University of Tsukuba, Ibaraki, Japan.
Fujishima Y
Corbell N
D'Souza C
Nakano M M
Zuber P
Yamane K
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.1.93
Published
1993-01-11
Pages
93-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC309069
Subset
IM
Grants
PHS HHS · D13262 · United States
NIGMS NIH HHS · GM39476 · United States
NIGMS NIH HHS · GM45898 · United States
Databases
GENBANK
D13262
External Links
PubMed source DOI: 10.1093/nar/21.1.93 PMC Full Text View journal details
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