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PMID: 8425043 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Differential expression of U5snRNA gene variants in maize (Zea mays) protoplasts.

Plant molecular biology ·Vol. 21 ·No. 1 ·1993-01-00 ·Pages 133-43

Leader D, Connelly S, Filipowicz W, Waugh R, Brown JW

Abstract

The small nuclear ribonucleoprotein particles U1, U2, U4/U6 and U5 participate in the removal of introns from pre-messenger RNAs in the nucleus. Three genes encoding U5snRNAs, the RNA moiety of U5snRNPs, have been isolated from maize. As in other plant UsnRNA gene families the three maize U5snRNA genes exhibit sequence variation. Two of the gene variants (MzU5.1 and MzU5.2) are clearly expressed after transfection into maize leaf protoplasts while the third gene variant (MzU5.3) is expressed at very low levels. These different levels of expression cannot be directly correlated with sequence changes in the highly conserved Upstream Sequence Element (USE) required for expression of Arabidopsis UsnRNA genes nor with differential stability of the U5snRNA transcripts. Further sequence elements may therefore have a role in regulating maize UsnRNA gene expression.

MeSH Terms
Base Sequence Chimera Cloning, Molecular Gene Expression Regulation Genes, Plant Genetic Variation Introns Molecular Sequence Data Nucleic Acid Conformation Promoter Regions, Genetic Protoplasts/physiology RNA Precursors/genetics RNA, Small Nuclear/genetics Sequence Homology, Nucleic Acid Transcription, Genetic Zea mays/genetics
Chemicals
RNA Precursors RNA, Small Nuclear
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Leader D
Scottish Crop Research Institute, Invergowrie, Dundee.
Connelly S
Filipowicz W
Waugh R
Brown J W
References (35)
35 references, click to expand
  1. Structure and expression of the U5 snRNA gene of Arabidopsis thaliana. Conserved upstream sequence elements in plant U-RNA genes.
    Nucleic Acids Res. 1988 Nov 25;16(22):10425-40 PMID: 3205713
  2. U6 snRNA genes of Arabidopsis are transcribed by RNA polymerase III but contain the same two upstream promoter elements as RNA polymerase II-transcribed U-snRNA genes.
    Nucleic Acids Res. 1990 Jun 25;18(12 ):3451-8 PMID: 2362802
  3. U1, U2 and U5 small nuclear RNAs are found in plants cells. Complete nucleotide sequence of the U5 RNA family from pea nuclei.
    Nucleic Acids Res. 1983 Dec 20;11(24):8583-94 PMID: 6200829
  4. Molecular analysis of a U3 RNA gene locus in tomato: transcription signals, the coding region, expression in transgenic tobacco plants and tandemly repeated pseudogenes.
    Nucleic Acids Res. 1990 Apr 25;18(8):1941-9 PMID: 2336383
  5. Amplification of plant U3 and U6 snRNA gene sequences using primers specific for an upstream promoter element and conserved intragenic regions.
    Nucleic Acids Res. 1990 Jun 25;18(12):3459-66 PMID: 2362803
  6. Cloning and characterisation of a U6 small nuclear RNA gene from potato.
    Biochim Biophys Acta. 1991 Dec 2;1129(1):90-2 PMID: 1836741
  7. Sequence variation and linkage of potato U2snRNA-encoding genes established by PCR.
    Gene. 1991 Nov 15;107(2):197-204 PMID: 1836193
  8. Different effects of intron nucleotide composition and secondary structure on pre-mRNA splicing in monocot and dicot plants.
    EMBO J. 1991 Sep;10(9):2635-44 PMID: 1868837
  9. Analysis of pre-mRNA processing in transfected plant protoplasts.
    Methods Enzymol. 1990;181:148-61 PMID: 1696344
  10. RNA-polymerase specificity of transcription of Arabidopsis U snRNA genes determined by promoter element spacing.
    Nature. 1990 Jul 12;346(6280):199-202 PMID: 2366873
  11. Nucleotide sequences of two soybean U1 snRNA genes.
    Nucleic Acids Res. 1988 May 11;16(9):4176 PMID: 3375086
  12. U5 small nuclear ribonucleoprotein: RNA structure analysis and ATP-dependent interaction with U4/U6.
    Mol Cell Biol. 1989 Aug;9(8):3350-9 PMID: 2552294
  13. Nucleotide sequence of a pea U2 snRNA gene.
    Nucleic Acids Res. 1989 Dec 11;17(23):10106 PMID: 2602118
  14. An essential yeast snRNA with a U5-like domain is required for splicing in vivo.
    Cell. 1987 Jun 5;49(5):613-24 PMID: 3555841
  15. Structure of U2 snRNA genes of Arabidopsis thaliana and their expression in electroporated plant protoplasts.
    EMBO J. 1988 Mar;7(3):791-9 PMID: 16453831
  16. Characterization of the U3 and U6 snRNA genes from wheat: U3 snRNA genes in monocot plants are transcribed by RNA polymerase III.
    Plant Mol Biol. 1992 Sep;19(6):973-83 PMID: 1511142
  17. Nucleotide sequence of a bean (Phaseolus vulgaris) U1 small nuclear RNA gene: implications for plant pre-mRNA splicing.
    Proc Natl Acad Sci U S A. 1987 Dec;84(24):9094-8 PMID: 3480532
  18. Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter.
    Nucleic Acids Res. 1984 Sep 25;12(18):7035-56 PMID: 6091052
  19. Maize U2 snRNAs: gene sequence and expression.
    Nucleic Acids Res. 1989 Nov 25;17(22):8991-9001 PMID: 2531369
  20. Gene splicing by overlap extension: tailor-made genes using the polymerase chain reaction.
    Biotechniques. 1990 May;8(5):528-35 PMID: 2357375
  21. High evolutionary conservation of the secondary structure and of certain nucleotide sequences of U5 RNA.
    Nucleic Acids Res. 1983 Dec 10;11(23):8359-67 PMID: 6200827
  22. Spliceosomal snRNAs.
    Annu Rev Genet. 1988;22:387-419 PMID: 2977088
  23. The 5' end of the coding region of a U6 RNA gene candidate from tomato starts with GUCC, a phylogenetically highly conserved 5' end sequence of U6 RNA.
    Nucleic Acids Res. 1990 Mar 11;18(5):1295 PMID: 2320424
  24. A U-snRNA gene-specific upstream element and a -30 'TATA box' are required for transcription of the U2 snRNA gene of Arabidopsis thaliana.
    EMBO J. 1989 Dec 1;8(12):3875-82 PMID: 2583119
  25. Characterization and expression of U1snRNA genes from potato.
    Plant Mol Biol. 1992 Sep;19(6):959-71 PMID: 1511141
  26. Structure and evolution of the U2 small nuclear RNA multigene family in primates: gene amplification under natural selection?
    Mol Cell Biol. 1990 Nov;10(11):5876-82 PMID: 2233721
  27. Improved free-energy parameters for predictions of RNA duplex stability.
    Proc Natl Acad Sci U S A. 1986 Dec;83(24):9373-7 PMID: 2432595
  28. Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information.
    Nucleic Acids Res. 1981 Jan 10;9(1):133-48 PMID: 6163133
  29. Molecular analysis of eight U1 RNA gene candidates from tomato that could potentially be transcribed into U1 RNA sequence variants differing from each other in similar regions of secondary structure.
    Nucleic Acids Res. 1989 Aug 11;17(15):6319-37 PMID: 2528122
  30. The AU-rich sequences present in the introns of plant nuclear pre-mRNAs are required for splicing.
    Cell. 1989 Aug 11;58(3):473-83 PMID: 2758463
  31. Monocot and dicot pre-mRNAs are processed with different efficiencies in transgenic tobacco.
    EMBO J. 1986 Oct;5(10):2419-25 PMID: 16453710
  32. Sequence and expression of potato U2 snRNA genes.
    Nucleic Acids Res. 1991 Jan 25;19(2):249-56 PMID: 2014165
  33. Nucleotide sequence and expression of a new Arabidopsis U2 snRNA gene.
    Nucleic Acids Res. 1990 Sep 11;18(17):5280 PMID: 2402448
  34. Alteration of the RNA polymerase specificity of U3 snRNA genes during evolution and in vitro.
    Cell. 1991 May 3;65(3):517-26 PMID: 1826860
  35. Purification of the major UsnRNPs from broad bean nuclear extracts and characterization of their protein constituents.
    Nucleic Acids Res. 1989 Feb 25;17(4):1445-58 PMID: 2522185
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00039624
Published
1993-01-00
Pages
133-43
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00039624 View journal details
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