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PMID: 2531369 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Maize U2 snRNAs: gene sequence and expression.

Nucleic acids research ·Vol. 17 ·No. 22 ·1989-11-25 ·Pages 8991-9001

Brown JW, Waugh R

Abstract

The complexity of plant U-type small nuclear ribonucleoprotein particles (UsnRNPs) may represent one level at which differences in splicing between animals and plants and between monocotyledonous and dicotyledonous plants could be effected. The maize (monocot.) U2snRNA multigene family consists of some 25 to 40 genes which from RNA blot and RNase protection analyses produce U2snRNAs varying in both size and sequence. The first 77 nucleotides of the maize U2-27 snRNA gene are identical to U2snRNA genes of Arabidopsis (dicot). Despite much lower sequence homology in the remaining 120 nucleotides the secondary structure of the RNA is conserved. The difference in splicing between monocot. and dicot. plants cannot be explained on the basis of sequence differences between monocot, and dicot. U2snRNAs in the region which may interact with intron branch point sequences.

MeSH Terms
Base Sequence Cloning, Molecular Gene Expression Genes Molecular Sequence Data Nucleic Acid Conformation Plants/genetics RNA Splicing RNA, Small Nuclear/genetics Restriction Mapping Ribonucleoproteins/genetics Ribonucleoproteins, Small Nuclear Transcription, Genetic Zea mays/genetics
Chemicals
RNA, Small Nuclear Ribonucleoproteins Ribonucleoproteins, Small Nuclear
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brown J W
Department of Biological Sciences, University of Dundee, UK.
Waugh R
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42 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/17.22.8991
Published
1989-11-25
Pages
8991-9001
Language
English
Region
England
NLM ID
0411011
PMCID
PMC335108
Subset
IM
Databases
GENBANK
X16459
External Links
PubMed source DOI: 10.1093/nar/17.22.8991 PMC Full Text View journal details
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