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PMID: 8413234 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Drosophila Polycomb-group gene Enhancer of zeste contains a region with sequence similarity to trithorax.

Molecular and cellular biology ·Vol. 13 ·No. 10 ·1993-10-00 ·Pages 6357-66

Jones RS, Gelbart WM

Abstract

As is typical of Polycomb-group loci, the Enhancer of zeste [E(z)] gene negatively regulates the segment identity genes of the Antennapedia (ANT-C) and Bithorax (BX-C) gene complexes. A second class of loci, collectively known as the trithorax group, plays an antagonistic role as positive regulators of the ANT-C and BX-C genes. Molecular analysis of the E(z) gene predicts a 760-amino-acid protein product. A region of 116 amino acids near the E(z) carboxy terminus is 41.2% identical (68.4% similar) with a carboxy-terminal region of the trithorax protein. This portion of the trithorax protein is part of a larger region previously shown to share extensive homology with a human protein (ALL-1/Hrx) that is implicated in acute leukemias. Over this same 116 amino acids, E(z) and ALL-1/Hrx are 43.9% identical (68.4% similar). Otherwise, E(z) is not significantly similar to any previously described proteins. As this region of sequence similarity is shared by two proteins with antagonistic functions, we suggest that it may comprise a domain that interacts with a common target, either nucleic acid or protein. Opposite effects on transcription might then be determined by other portions of the two proteins.

Related Genes
E(z) trx
MeSH Terms
Alleles Amino Acid Sequence Animals Base Sequence Blotting, Northern Chromosome Walking Cloning, Molecular DNA DNA-Binding Proteins/genetics Drosophila/genetics,growth & development Drosophila Proteins Gene Expression Regulation Genes, Regulator Humans Molecular Sequence Data Mutation Nuclear Proteins Phenotype Polycomb Repressive Complex 2 Proteins/genetics Repressor Proteins Restriction Mapping Sequence Homology, Amino Acid Transcription Factors
Chemicals
DNA-Binding Proteins Drosophila Proteins Nuclear Proteins Proteins Repressor Proteins Transcription Factors Trl protein, Drosophila DNA E(z) protein, Drosophila Polycomb Repressive Complex 2
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Jones R S
Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138-2097.
Gelbart W M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.10.6357-6366.1993
Published
1993-10-00
Pages
6357-66
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC364694
Subset
IM
Databases
GENBANK
U00180
External Links
PubMed source DOI: 10.1128/mcb.13.10.6357-6366.1993 PMC Full Text View journal details
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