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PMID: 8400140 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A survey of transcripts expressed specifically in root nodules of broadbean (Vicia faba L.).

Plant molecular biology ·Vol. 22 ·No. 6 ·1993-09-00 ·Pages 957-70

Perlick AM, Pühler A

Abstract

More than 600 potentially nodule-specific clones have been detected by differential hybridization of a broadbean cDNA library constructed from root nodule poly(A)+ RNA. These isolated cDNAs belong to at least 28 different clone groups containing cross-hybridizing sequences. The number of clones within a clone group varies from about 200 to only one single clone. Northern hybridization experiments revealed nodule-specific transcripts for 14 clone groups and markedly nodule-enhanced transcripts for another 7 clone groups. Sequence homologies indicate that three transcript sequences code for different leghemoglobins. Two other transcripts encode a nodule-specific sucrose synthase and a nodule-enhanced asparagine synthetase, respectively. Four deduced gene products are proline-rich, two of them being the homologues of PsENOD2 and PsENOD12. The third proline-rich protein (PRP) is composed of similar amino acid repeats as the nodule-specific PsENOD12 but is expressed in nodules and roots in comparable amounts. The fourth PRP is a nodule-enhanced extensin-type protein built up by Ser-Pro4 repeats. Two further nodule-specific transcripts encode gene products showing some similarity to structural glycine-rich proteins. Additionally, transcripts could be identified for broadbean homologues of the nodulins MsNOD25, PsENOD3 and PsENOD5 and transcripts specifying a nodule-enhanced lipoxygenase and a translation elongation factor EF-1 alpha, which is expressed in all broadbean tissues tested.

MeSH Terms
Amino Acid Sequence Blotting, Northern Cloning, Molecular DNA, Complementary Fabaceae/anatomy & histology,genetics Gene Library Lipoxygenase/genetics Molecular Sequence Data Nitrogen Fixation/genetics Peptide Elongation Factor 1 Peptide Elongation Factors/genetics Peptides/genetics Plant Proteins/genetics Plants, Medicinal Proline-Rich Protein Domains RNA, Messenger/genetics Transcription, Genetic
Chemicals
DNA, Complementary Peptide Elongation Factor 1 Peptide Elongation Factors Peptides Plant Proteins RNA, Messenger Lipoxygenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Perlick A M
Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld, Germany.
Pühler A
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Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00028969
Published
1993-09-00
Pages
957-70
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00028969 View journal details
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