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PMID: 2515855 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

The cDNA cloning of a pea (Pisum sativum) seed lipoxygenase. Sequence comparisons of the two major pea seed lipoxygenase isoforms.

The Biochemical journal ·Vol. 264 ·No. 3 ·1989-12-15 ·Pages 929-32

Ealing PM, Casey R

Abstract

Cloning and sequencing of two cDNAs from mRNA of maturing pea (Pisum sativum) seeds allowed the deduction of the complete amino acid sequence of a lipoxygenase polypeptide which is most similar to that of soya-bean lipoxygenase 2. The predicted Mr of this polypeptide is 97134, and its sequence permits comparisons between the lox2-type and the lox3-type lipoxygenase isoforms from pea and soya bean (Glycine max).

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA/genetics,isolation & purification Fabaceae/enzymology,genetics Genes, Plant Isoenzymes/genetics Lipoxygenase/genetics Molecular Sequence Data Plants, Medicinal Protein Biosynthesis Seeds/enzymology Sequence Homology, Nucleic Acid
Chemicals
Isoenzymes DNA Lipoxygenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ealing P M
John Innes Institute, Norwich, U.K.
Casey R
References (10)
10 references, click to expand
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    Biochem J. 1985 Nov 15;232(1):79-85 PMID: 2417590
  5. The complete sequence of the rabbit erythroid cell-specific 15-lipoxygenase mRNA: comparison of the predicted amino acid sequence of the erythrocyte lipoxygenase with other lipoxygenases.
    Gene. 1989 Jun 30;79(1):181-8 PMID: 2777088
  6. Cloning of the cDNA for human 5-lipoxygenase.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):416-20 PMID: 3422434
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    J Biol Chem. 1988 May 15;263(14):6816-21 PMID: 2834391
  8. The complete amino acid sequence of a pea (Pisum sativum) seed lipoxygenase predicted from a near full-length cDNA.
    Biochem J. 1988 Aug 1;253(3):915-8 PMID: 3140791
  9. Molecular cloning and primary structure of human 15-lipoxygenase.
    Biochem Biophys Res Commun. 1988 Dec 15;157(2):457-64 PMID: 3202857
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2640929
Published
1989-12-15
Pages
929-32
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1133675
Subset
IM
Databases
GENBANK
X17061
External Links
PubMed source DOI: 10.1042/bj2640929 PMC Full Text View journal details
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