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PMID: 3140791 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

The complete amino acid sequence of a pea (Pisum sativum) seed lipoxygenase predicted from a near full-length cDNA.

The Biochemical journal ·Vol. 253 ·No. 3 ·1988-08-01 ·Pages 915-8

Ealing PM, Casey R

Abstract

A near full-length cDNA for a pea (Pisum sativum) seed lipoxygenase was isolated and sequenced. It has a protein coding sequence (2583 bp), 5' (59 bp) and 3' (191 bp) non-coding regions, and a poly(A) tail (20 bp). The predicted amino acid sequence indicates a polypeptide of Mr 97,628 that shows about 86% amino acid identity with a soya-bean lipoxygenase 3 protein sequence [Yenofsky, Fine & Liu (1988) Mol. Gen. Genet. 211, 215-222]. The cDNA directs the transcription of mRNA that can be translated to give an anti-lipoxygenase-precipitable polypeptide in vitro.

MeSH Terms
Amino Acid Sequence Base Sequence DNA/genetics Electrophoresis, Polyacrylamide Gel Fabaceae/enzymology Lipoxygenase/biosynthesis,genetics Molecular Sequence Data Plants, Medicinal Protein Biosynthesis RNA, Messenger/genetics Seeds/enzymology Transcription, Genetic
Chemicals
RNA, Messenger DNA Lipoxygenase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Ealing P M
John Innes Institute, Norwich, U.K.
Casey R
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14 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2530915
Published
1988-08-01
Pages
915-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1149390
Subset
IM
Databases
GENBANK
X07807
External Links
PubMed source DOI: 10.1042/bj2530915 PMC Full Text View journal details
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