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PMID: 8397216 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Three-dimensional solution structure of Acanthamoeba profilin-I.

The Journal of cell biology ·Vol. 122 ·No. 6 ·1993-09-00 ·Pages 1277-83

Vinson VK, Archer SJ, Lattman EE, Pollard TD, Torchia DA

Abstract

We have determined a medium resolution three-dimensional solution structure of Acanthamoeba profilin-I by multidimensional nuclear magnetic resonance spectroscopy. This 13-kD actin binding protein consists of a five stranded antiparallel beta sheet flanked by NH2- and COOH-terminal helices on one face and by a third helix and a two stranded beta sheet on the other face. Data from actin-profilin cross-linking experiments and the localization of conserved residues between profilins in different phyla indicate that actin binding occurs on the molecular face occupied by the terminal helices. The other face of the molecule contains the residues that differ between Acanthamoeba profilins-I and II and may be important in determining the difference in polyphosphoinositide binding between these isoforms. This suggests that lipids and actin bind to different faces of the molecule.

MeSH Terms
Acanthamoeba/chemistry,metabolism Actins/metabolism Amino Acid Sequence Animals Contractile Proteins Image Processing, Computer-Assisted Isomerism Magnetic Resonance Spectroscopy Microfilament Proteins/chemistry Molecular Sequence Data Phosphatidylinositol Phosphates Phosphatidylinositols/metabolism Profilins Solutions
Chemicals
Actins Contractile Proteins Microfilament Proteins Phosphatidylinositol Phosphates Phosphatidylinositols Profilins Solutions
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Vinson V K
Department of Cell Biology and Anatomy, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Archer S J
Lattman E E
Pollard T D
Torchia D A
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31 references, click to expand
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.122.6.1277
Published
1993-09-00
Pages
1277-83
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2119855
Subset
IM
Grants
NIGMS NIH HHS · GM-35171 · United States
NIGMS NIH HHS · GM13620 · United States
External Links
PubMed source DOI: 10.1083/jcb.122.6.1277 PMC Full Text View journal details
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