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PMID: 8388872 Published · ppublish English Comparative Study Journal Article

Cloning, sequencing, and expression of the DNA gyrase genes from Staphylococcus aureus.

Journal of bacteriology ·Vol. 175 ·No. 11 ·1993-06-00 ·Pages 3269-77

Brockbank SM, Barth PT

Abstract

We have isolated and cloned the gyrA and gyrB genes from Staphylococcus aureus. These adjacent genes encode the subunits of DNA gyrase. The nucleotide sequence of a 5.9-kb region which includes part of an upstream recF gene, the whole of gyrB and gyrA, and about 1 kb of unknown downstream sequence has been determined. The gyrB and gyrA gene sequences predict proteins of 886 and 644 amino acid residues, respectively, which have significant homologies with the gyrase subunits of Escherichia coli and Bacillus subtilis. Residues thought to be important to the structure and function of the subunits are conserved. These genes have been expressed separately by using a T7 promoter vector. N-terminal sequencing of the cloned gene products suggests that the mature GyrB subunit exists mainly with its initial five residues removed. Protein sequencing also supports the interpretation of our DNA sequencing data, which are inconsistent in several placed with the recently published sequence of the same genes (E. E. C. Margerrison, R. Hopewell, and L. M. Fisher, J. Bacteriol. 174:1596-1603, 1992).

Related Genes
gyrA gyrB
MeSH Terms
Amino Acid Sequence Artifacts Base Sequence Cloning, Molecular DNA Gyrase DNA Topoisomerases, Type II/biosynthesis,genetics Genes, Bacterial/genetics Molecular Sequence Data Recombinant Proteins/biosynthesis Restriction Mapping Sequence Analysis, DNA Sequence Homology, Amino Acid Staphylococcus aureus/enzymology,genetics
Chemicals
Recombinant Proteins DNA Gyrase DNA Topoisomerases, Type II
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Brockbank S M
Department of Infection Research, Zeneca Pharmaceuticals, Alderley Park, Macclesfield, Cheshire, United Kingdom.
Barth P T
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38 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.11.3269-3277.1993
Published
1993-06-00
Pages
3269-77
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC204723
Subset
IM
Databases
GENBANK
X71437
External Links
PubMed source DOI: 10.1128/jb.175.11.3269-3277.1993 PMC Full Text View journal details
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