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PMID: 8388482 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Biochemical and genetic evidence for a pseudoknot structure at the 3' terminus of the poliovirus RNA genome and its role in viral RNA amplification.

Journal of virology ·Vol. 67 ·No. 6 ·1993-06-00 ·Pages 2961-71

Jacobson SJ, Konings DA, Sarnow P

Abstract

The sequences in the plus-stranded poliovirus RNA genome that dictate the specific amplification of viral RNA in infected cells remain unknown. We have analyzed the structure of the 3' noncoding region of the viral genome by thermodynamic-based structure calculation and by chemical and enzymatic probing of in vitro-synthesized RNAs and provide evidence for the existence of an RNA pseudoknot structure in this region. To explore the functional significance of this structure, revertants of a mutant bearing a lesion in the proposed pseudoknot and exhibiting a temperature-sensitive defect in viral RNA synthesis were isolated and mapped. The results of this genetic analysis established a correlation between the structure of the 3' terminus of the viral RNA and its function in vivo in RNA amplification. Furthermore, phylogenetic analysis indicated that a similar structure could be formed in coxsackievirus B1, a related enterovirus, which further supports a role for the pseudoknot structure in viral RNA amplification in infected cells.

MeSH Terms
Base Sequence Computer Simulation Enterovirus B, Human/genetics Gene Amplification/genetics Genome, Viral HeLa Cells Humans Molecular Sequence Data Mutagenesis, Site-Directed Nucleic Acid Conformation Phylogeny Picornaviridae/genetics Poliovirus/genetics,growth & development RNA, Viral/biosynthesis Regulatory Sequences, Nucleic Acid/genetics Thermodynamics Virus Replication/genetics
Chemicals
RNA, Viral
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Jacobson S J
Department of Biochemistry, Biophysics and Genetics, University of Colorado Health Sciences Center, Denver 80262.
Konings D A
Sarnow P
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.6.2961-2971.1993
Published
1993-06-00
Pages
2961-71
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237632
Subset
IM
Grants
NIAID NIH HHS · AI-25105 · United States
NINDS NIH HHS · NS-07321 · United States
External Links
PubMed source DOI: 10.1128/JVI.67.6.2961-2971.1993 PMC Full Text View journal details
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