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PMID: 8386900 Published · ppublish English Journal Article

Effects of hyaluronic acid on macrophage phagocytosis and active oxygen release.

Agents and actions ·Vol. 38 ·No. 1-2 ·1993-01-00 ·Pages 32-7

Suzuki Y, Yamaguchi T

Abstract

The effects of hyaluronic acid (HA) on macrophage function, in terms of the phagocytosis of latex beads and superoxide anion and hydrogen peroxide release stimulated by phorbol myristate acetate (PMA), were studied in guinea-pig peritoneal macrophages. Phagocytosis was inhibited in a dose- and molecular-weight-dependent manner by HA. The addition of PMA to the culture, at a dose of more than 10 ng ml-1, caused an increase in the release of active oxygens. The release of active oxygens was inhibited by high molecular weight HA (MW 2.02 x 10(6), HA-202) in a dose-dependent manner. In cell-free systems, HA-202 had a negligible effect in scavenging these active oxygens. Of the three molecular sizes of HA (MW: 0.28 x 10(6), 0.98 x 10(6) and 2.02 x 10(6)), HA-202 most strongly inhibited the active oxygen release. These results indicate that high-molecular-weight HA acts directly on macrophages to inhibit phagocytosis and active oxygen formation, which, in turn, ameliorates the progression of chronic inflammation.

MeSH Terms
Animals Cells, Cultured Dose-Response Relationship, Drug Guinea Pigs Hyaluronic Acid/pharmacology Hydrogen Peroxide/metabolism Macrophages/drug effects,immunology Male Molecular Weight Peritoneal Cavity Phagocytosis/drug effects Plastic Embedding Reactive Oxygen Species/metabolism Superoxides/metabolism Tetradecanoylphorbol Acetate/pharmacology Xanthine Xanthine Oxidase/metabolism Xanthines/metabolism
Chemicals
Reactive Oxygen Species Xanthines Superoxides Xanthine Hyaluronic Acid Hydrogen Peroxide Xanthine Oxidase Tetradecanoylphorbol Acetate
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Suzuki Y
Shiseido Pharmaceutical Research Laboratories, Yokohama, Japan.
Yamaguchi T
References (17)
17 references, click to expand
  1. The interaction of hyaluronate with the cell surface: the hyaluronate receptor and the core protein.
    Ciba Found Symp. 1989;143:87-99; discussion 100-6, 281-5 PMID: 2478346
  2. Oxygen derived free radicals and synovial fluid hyaluronate.
    Ann Rheum Dis. 1991 Jun;50(6):389-92 PMID: 1711835
  3. Hydrogen peroxide suppresses the proteoglycan synthesis of intact articular cartilage.
    J Rheumatol. 1985 Apr;12(2):205-10 PMID: 3928892
  4. Increased superoxide anion production by immunologically activated and chemically elicited macrophages.
    J Exp Med. 1978 Jul 1;148(1):115-27 PMID: 209122
  5. Generation of superoxide anion and chemiluminescence by human monocytes during phagocytosis and on contact with surface-bound immunoglobulin G.
    J Exp Med. 1976 Jun 1;143(6):1551-6 PMID: 178824
  6. The synovial membrane in osteoarthritis: a histological study including the characterisation of the cellular infiltrate present in inflammatory osteoarthritis using monoclonal antibodies.
    Ann Rheum Dis. 1988 Apr;47(4):300-7 PMID: 3259125
  7. Oxygen free radicals, inflammation, and synovitis: and synovitis: the current status.
    Ann Rheum Dis. 1989 Oct;48(10):864-70 PMID: 2684056
  8. Inhibition of phagocytosis by high molecular weight hyaluronate.
    Immunology. 1980 Jul;40(3):435-46 PMID: 7429537
  9. Concentration and molecular weight of sodium hyaluronate in synovial fluid from patients with rheumatoid arthritis and other arthropathies.
    Ann Rheum Dis. 1985 Dec;44(12):817-22 PMID: 4083937
  10. Oxygen free-radicals mediate an inhibition of proteoglycan synthesis in cultured articular cartilage.
    Ann Rheum Dis. 1984 Jun;43(3):462-9 PMID: 6331328
  11. The mechanism of chondrocyte hydrogen peroxide damage. Depletion of intracellular ATP due to suppression of glycolysis caused by oxidation of glyceraldehyde-3-phosphate dehydrogenase.
    J Rheumatol. 1989 Jan;16(1):7-14 PMID: 2716009
  12. Changes in the viscosity of hyaluronic acid after exposure to a myeloperoxidase-derived oxidant.
    Arthritis Rheum. 1989 Apr;32(4):461-7 PMID: 2539826
  13. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
    Anal Biochem. 1976 May 7;72:248-54 PMID: 942051
  14. A semi-automated micro-assay for H2O2 release by human blood monocytes and mouse peritoneal macrophages.
    J Immunol Methods. 1985 Apr 22;78(2):323-36 PMID: 3989315
  15. Hyaluronic acid synthesis in articular cartilage: an inhibition by hydrogen peroxide.
    Biochem Biophys Res Commun. 1985 Oct 30;132(2):714-20 PMID: 3840689
  16. Candidacidal activity of monocyte-derived human macrophages: relationship between Candida killing and oxygen radical generation by human macrophages.
    J Leukoc Biol. 1987 Apr;41(4):289-94 PMID: 3033107
  17. Antioxidant activity of synovial fluid, hyaluronic acid, and two subcomponents of hyaluronic acid. Synovial fluid scavenging effect is enhanced in rheumatoid arthritis patients.
    Arthritis Rheum. 1988 Jan;31(1):63-71 PMID: 3345232
Article Info
Journal
Agents and actions
Abbr.
Agents Actions
ISSN
0065-4299
DOI
10.1007/BF02027210
Published
1993-01-00
Pages
32-7
Language
English
Region
Switzerland
NLM ID
0213341
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02027210
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