Oxygen free-radicals mediate an inhibition of proteoglycan synthesis in cultured articular cartilage.

Annals of the rheumatic diseases ·Vol. 43 ·No. 3 ·1984-06-00 ·Pages 462-9

Bates EJ, Lowther DA, Handley CJ

Abstract

Superoxide radical, generated enzymatically by the action of xanthine oxidase on hypoxanthine, significantly inhibited proteoglycan synthesis by cultured bovine articular cartilage. This inhibition was not due to the generation of uric acid or to the generation of superoxide per se. It was immediate in onset and still evident after six days in culture. The inhibition was similar for both 35S-sulphate and 3H-acetate incorporation into glycosaminoglycans and could not be reversed by addition of beta-D-benzyl xyloside. Protein synthesis was also inhibited.

MeSH Terms

Animals Cartilage, Articular/drug effects,metabolism Cattle Culture Techniques Free Radicals Glycosaminoglycans/biosynthesis Glycosides/pharmacology Hypoxanthine Hypoxanthines/pharmacology Protein Biosynthesis Proteoglycans/biosynthesis Superoxide Dismutase/pharmacology Superoxides/pharmacology Uric Acid/pharmacology Xanthine Oxidase/pharmacology

Chemicals

Free Radicals Glycosaminoglycans Glycosides Hypoxanthines Proteoglycans xylosides Superoxides Uric Acid Hypoxanthine Superoxide Dismutase Xanthine Oxidase

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Bates E J

Lowther D A

Handley C J

References (36)

36 references, click to expand

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Article Info

Journal

Annals of the rheumatic diseases

Abbr.

Ann Rheum Dis

ISSN

0003-4967

DOI

10.1136/ard.43.3.462

Published

1984-06-00

Pages

462-9

Language

English

Region

England

NLM ID

0372355

PMCID

PMC1001371

Subset

External Links

PubMed source DOI: 10.1136/ard.43.3.462 PMC Full Text View journal details

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Oxygen free-radicals mediate an inhibition of proteoglycan synthesis in cultured articular cartilage.

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