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PMID: 8384310 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Mutations in the Drosophila gene encoding ribosomal protein S6 cause tissue overgrowth.

Molecular and cellular biology ·Vol. 13 ·No. 4 ·1993-04-00 ·Pages 2524-35

Stewart MJ, Denell R

Abstract

We have characterized two P-element-induced, lethal mutations in Drosophila melanogaster which affect the larval hemocytes, mediators of the insect immune response. Each mutant displays larval melanotic tumors characteristic of mutations affecting the insect cellular immune system, and the moribund animals develop grossly hypertrophied hematopoietic organs because of increased cell proliferation and extra rounds of endoreduplication in some hematopoietic cells. Surprisingly, these mutations are due to P element insertions in the 5' regulatory region of the Drosophila gene encoding ribosomal protein S6 and cause a reduction of S6 transcript abundance in mutant larvae.

Related Genes
S6 bys ct sn
MeSH Terms
Animals Base Sequence Chromosome Mapping Cloning, Molecular DNA Transposable Elements Drosophila melanogaster/cytology,genetics Gene Expression Gene Expression Regulation Genes, Insect Genes, Lethal Hematopoietic Stem Cells/physiology Humans In Situ Hybridization Molecular Sequence Data Mutagenesis, Insertional Mutation Oligodeoxyribonucleotides/chemistry RNA, Messenger/genetics Rats Restriction Mapping Ribosomal Protein S6 Ribosomal Proteins/genetics Sequence Alignment X Chromosome
Chemicals
DNA Transposable Elements Oligodeoxyribonucleotides RNA, Messenger Ribosomal Protein S6 Ribosomal Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Stewart M J
Center for Basic Cancer Research, Kansas State University, Manhattan 66506.
Denell R
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37 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.13.4.2524-2535.1993
Published
1993-04-00
Pages
2524-35
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC359579
Subset
IM
Databases
GENBANK
L0274, L0275, L0276
External Links
PubMed source DOI: 10.1128/mcb.13.4.2524-2535.1993 PMC Full Text View journal details
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