Home LiteratureArticle Details
PMID: 8382292 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Pseudorabies virus protein homologous to herpes simplex virus type 1 ICP18.5 is necessary for capsid maturation.

Journal of virology ·Vol. 67 ·No. 3 ·1993-03-00 ·Pages 1236-45

Mettenleiter TC, Saalmüller A, Weiland F

Abstract

In pseudorabies virus (PrV), an open reading frame that partially overlaps the gene for the essential glycoprotein gII has been shown to encode a protein homologous to the ICP18.5 polypeptide of herpes simplex virus type 1 (N. Pederson and L. Enquist, Nucleic Acids Res. 17:3597, 1989). To study the function of this protein during the viral replicative cycle, a PrV mutant which carries a beta-galactosidase expression cassette interrupting the ICP18.5(PrV) gene was constructed. This mutant could be propagated only on cell lines that were able to provide ICP18.5(PrV) in trans after transformation with a corresponding genomic PrV DNA fragment. Detailed analysis showed that inactivation of the ICP18.5(PrV) gene did not impair infection of noncomplementing cells, nor did it impair early or late gene expression, as shown by immunoprecipitation of glycoproteins gII, gIII, and gp50. Surface localization of glycoproteins as demonstrated by fluorescence-activated cell sorting analyses was also not affected. Southern blot hybridizations, however, showed that cleavage of replicative concatemeric viral DNA did not occur in noncomplementing cells infected by the ICP18.5 mutant PrV. In addition, electron microscopic analysis revealed an accumulation of empty capsids in the nucleus of mutant-infected noncomplementing cells. We conclude that the ICP18.5(PrV) protein is necessary for viral replication and plays an essential role in the process of mature capsid formation.

MeSH Terms
Animals Capsid/biosynthesis DNA, Viral/metabolism DNA-Binding Proteins/genetics Genetic Complementation Test Glycoproteins/biosynthesis Herpesvirus 1, Suid/genetics,growth & development,ultrastructure Mutagenesis, Insertional Sequence Homology, Amino Acid Vero Cells Viral Envelope Proteins/biosynthesis,genetics Viral Proteins/biosynthesis,genetics Virus Replication
Chemicals
DNA, Viral DNA-Binding Proteins Glycoproteins ICP8 protein, Simplexvirus Viral Envelope Proteins Viral Proteins pseudorabies virus glycoproteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mettenleiter T C
Federal Research Center for Virus Diseases of Animals, Tübingen, Germany.
Saalmüller A
Weiland F
References (45)
45 references, click to expand
  1. Conservation of a gene cluster including glycoprotein B in bovine herpesvirus type 2 (BHV-2) and herpes simplex virus type 1 (HSV-1).
    Virology. 1988 Aug;165(2):388-405 PMID: 2841793
  2. cis Functions involved in replication and cleavage-encapsidation of pseudorabies virus.
    J Virol. 1986 Aug;59(2):318-27 PMID: 3016300
  3. Overexpression in bacterial and identification in infected cells of the pseudorabies virus protein homologous to herpes simplex virus type 1 ICP18.5.
    J Virol. 1991 Jul;65(7):3746-58 PMID: 1645790
  4. The nucleotide sequence of the glycoprotein gB gene of infectious laryngotracheitis virus: analysis and evolutionary relationship to the homologous gene from other herpesviruses.
    J Gen Virol. 1991 Feb;72 ( Pt 2):393-8 PMID: 1847176
  5. Transcription initiation sites and nucleotide sequence of a herpes simplex virus 1 gene conserved in the Epstein-Barr virus genome and reported to affect the transport of viral glycoproteins.
    J Virol. 1986 Dec;60(3):1134-40 PMID: 3023664
  6. Pseudorabies virus glycoproteins gII and gp50 are essential for virus penetration.
    J Virol. 1991 Oct;65(10):5348-56 PMID: 1654444
  7. The nucleotide sequence of the gB glycoprotein gene of HSV-2 and comparison with the corresponding gene of HSV-1.
    Virology. 1986 Dec;155(2):322-33 PMID: 3024391
  8. Herpes simplex virus amplicon: cleavage of concatemeric DNA is linked to packaging and involves amplification of the terminally reiterated a sequence.
    J Virol. 1986 Mar;57(3):933-41 PMID: 3005637
  9. Proteins specified by herpes simplex virus. 8. Characterization and composition of multiple capsid forms of subtypes 1 and 2.
    J Virol. 1972 Nov;10(5):1044-52 PMID: 4344252
  10. Structural organization of the conserved gene block of Herpesvirus saimiri coding for DNA polymerase, glycoprotein B, and major DNA binding protein.
    Virology. 1990 Feb;174(2):533-42 PMID: 2154888
  11. Identification and nucleotide sequence of a gene in equine herpesvirus 1 analogous to the herpes simplex virus gene encoding the major envelope glycoprotein gB.
    J Gen Virol. 1989 Feb;70 ( Pt 2):383-94 PMID: 2543744
  12. Isolation, characterization, and physical mapping of a pseudorabies virus mutant containing antigenically altered gp50.
    J Virol. 1984 Jul;51(1):57-62 PMID: 6202885
  13. Nucleotide sequence and characterization of the Marek's disease virus homologue of glycoprotein B of herpes simplex virus.
    J Gen Virol. 1989 Jul;70 ( Pt 7):1789-804 PMID: 2544666
  14. Characterization of a major DNA-binding domain in the herpes simplex virus type 1 DNA-binding protein (ICP8).
    J Virol. 1990 May;64(5):2082-9 PMID: 2157871
  15. Physical and genetic analysis of the herpes simplex virus DNA polymerase locus.
    Virology. 1980 Jun;103(2):311-26 PMID: 6247818
  16. Pseudorabies virus mutants lacking the essential glycoprotein gII can be complemented by glycoprotein gI of bovine herpesvirus 1.
    J Virol. 1991 Feb;65(2):621-31 PMID: 1846188
  17. Herpes simplex virus type 1 UL28 gene product is important for the formation of mature capsids.
    J Gen Virol. 1990 Oct;71 ( Pt 10):2377-84 PMID: 2172450
  18. Demonstration of three major species of pseudorabies virus glycoproteins and identification of a disulfide-linked glycoprotein complex.
    J Virol. 1985 Jan;53(1):166-73 PMID: 2578194
  19. Studies on the morphogenesis of murine cytomegalovirus.
    Intervirology. 1986;26(4):192-201 PMID: 3038772
  20. Expression of herpes simplex virus type 1 major DNA-binding protein, ICP8, in transformed cell lines: complementation of deletion mutants and inhibition of wild-type virus.
    J Virol. 1987 Apr;61(4):1136-46 PMID: 3029408
  21. Correct intranuclear localization of herpes simplex virus DNA polymerase requires the viral ICP8 DNA-binding protein.
    J Virol. 1991 Mar;65(3):1082-9 PMID: 1847437
  22. Identification and nucleotide sequence of the glycoprotein gB gene of equine herpesvirus 4.
    J Virol. 1989 Mar;63(3):1123-33 PMID: 2915378
  23. Herpesvirus (pseudorabies virus) latency in swine: occurrence and physical state of viral DNA in neural tissues.
    Virology. 1986 Dec;155(2):600-13 PMID: 3024403
  24. Characterization of a pseudorabies virus glycoprotein gene with homology to herpes simplex virus type 1 and type 2 glycoprotein C.
    J Virol. 1986 May;58(2):339-47 PMID: 3009851
  25. The complete DNA sequence of the long unique region in the genome of herpes simplex virus type 1.
    J Gen Virol. 1988 Jul;69 ( Pt 7):1531-74 PMID: 2839594
  26. Comparison of the bovine herpesvirus 1 gI gene and the herpes simplex virus type 1 gB gene.
    J Virol. 1988 Sep;62(9):3319-27 PMID: 2841484
  27. The nucleotide sequence of the herpes simplex virus type 1 late gene ICP18.5 of strain Angelotti.
    Nucleic Acids Res. 1987 Oct 12;15(19):8109-10 PMID: 2823222
  28. Transcriptional and genetic analyses of the herpes simplex virus type 1 genome: coordinates 0.29 to 0.45.
    J Virol. 1984 Mar;49(3):947-59 PMID: 6199514
  29. Genetic and phenotypic analysis of herpes simplex virus type 1 mutants conditionally resistant to immune cytolysis.
    J Virol. 1983 Sep;47(3):568-85 PMID: 6312082
  30. Role of glycoprotein B of herpes simplex virus type 1 in viral entry and cell fusion.
    J Virol. 1988 Aug;62(8):2596-604 PMID: 2839688
  31. DNA sequence and expression of the B95-8 Epstein-Barr virus genome.
    Nature. 1984 Jul 19-25;310(5974):207-11 PMID: 6087149
  32. Analysis of the protein-coding content of the sequence of human cytomegalovirus strain AD169.
    Curr Top Microbiol Immunol. 1990;154:125-69 PMID: 2161319
  33. A comparison of herpes simplex and pseudorabies viruses.
    Virology. 1959 Apr;7(4):394-407 PMID: 13669311
  34. The complete DNA sequence of varicella-zoster virus.
    J Gen Virol. 1986 Sep;67 ( Pt 9):1759-816 PMID: 3018124
  35. Characterization of the envelope proteins of pseudorabies virus.
    J Virol. 1984 Nov;52(2):583-90 PMID: 6092679
  36. Monoclonal antibodies reactive with swine lymphocytes. I. Antibodies to membrane structures that define the cytolytic T lymphocyte subset in the swine.
    J Immunol. 1984 Aug;133(2):647-52 PMID: 6610707
  37. Conservation of gene organization in the lymphotropic herpesviruses herpesvirus Saimiri and Epstein-Barr virus.
    J Virol. 1988 Mar;62(3):757-67 PMID: 2828671
  38. Transformation of mammalian cells to antibiotic resistance with a bacterial gene under control of the SV40 early region promoter.
    J Mol Appl Genet. 1982;1(4):327-41 PMID: 6286831
  39. A glycoprotein gX-beta-galactosidase fusion gene as insertional marker for rapid identification of pseudorabies virus mutants.
    J Virol Methods. 1990 Oct;30(1):55-65 PMID: 1964940
  40. Replication of herpesvirus DNA. V. Maturation of concatemeric DNA of pseudorabies virus to genome length is related to capsid formation.
    J Virol. 1980 Mar;33(3):1151-64 PMID: 6245265
  41. Herpes simplex-1 DNA polymerase. Identification of an intrinsic 5'----3' exonuclease with ribonuclease H activity.
    J Biol Chem. 1989 Nov 15;264(32):19266-70 PMID: 2553735
  42. Potential role for herpes simplex virus ICP8 DNA replication protein in stimulation of late gene expression.
    J Virol. 1991 May;65(5):2666-75 PMID: 1850040
  43. The nucleotide sequence of a pseudorabies virus gene similar to ICP18.5 of herpes simplex virus type 1.
    Nucleic Acids Res. 1989 May 11;17(9):3597 PMID: 2542904
  44. The pseudorabies virus gII gene is closely related to the gB glycoprotein gene of herpes simplex virus.
    J Virol. 1987 Sep;61(9):2691-701 PMID: 3039163
  45. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.67.3.1236-1245.1993
Published
1993-03-00
Pages
1236-45
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC237489
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.67.3.1236-1245.1993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2