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PMID: 3005637 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Herpes simplex virus amplicon: cleavage of concatemeric DNA is linked to packaging and involves amplification of the terminally reiterated a sequence.

Journal of virology ·Vol. 57 ·No. 3 ·1986-03-00 ·Pages 933-41

Deiss LP, Frenkel N

Abstract

Herpes simplex virus-infected cells contain large concatemeric DNA molecules arising from replication of the viral genome. The large concatemers are cleaved to generate unit-length molecules terminating at both ends with the a sequence. We have used constructed defective virus vectors (amplicons) derived from herpes simplex virus to study the mechanism of cleavage of viral DNA concatemers and the packaging of viral DNA into nucleocapsids. These studies revealed that (i) a 248-base-pair a sequence contained the signal(s) required for cleavage-packaging, (ii) the cleavage of viral DNA concatemers was coupled to packaging, (iii) the a sequence contained the information required for its own amplification, and (iv) cleavage-packaging occurred by a novel process involving the amplification of the a sequence.

MeSH Terms
Animals DNA Replication DNA, Viral/metabolism Gene Amplification Genes, Viral Plasmids Rabbits Repetitive Sequences, Nucleic Acid Simplexvirus/genetics Virus Replication
Chemicals
DNA, Viral
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Deiss L P
Frenkel N
References (28)
28 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.57.3.933-941.1986
Published
1986-03-00
Pages
933-41
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252824
Subset
IM
Grants
NIAID NIH HHS · AI-15488 · United States
NCI NIH HHS · CA-09241 · United States
NCI NIH HHS · CA-19264 · United States
External Links
PubMed source DOI: 10.1128/JVI.57.3.933-941.1986 PMC Full Text View journal details
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