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PMID: 8382024 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Detection of enteric viruses in oysters by using the polymerase chain reaction.

Applied and environmental microbiology ·Vol. 59 ·No. 2 ·1993-02-00 ·Pages 631-5

Atmar RL, Metcalf TG, Neill FH, Estes MK

Abstract

A procedure for the detection of enteric viral nucleic acid in oysters by the polymerase chain reaction was developed. Known quantities of poliovirus type 1 were seeded into oysters. Virus was extracted and concentrated by using organic flocculation and polyethylene glycol precipitation. Inhibitors of reverse transcription-polymerase chain reaction were present in the oyster extracts, preventing amplification of target viral nucleic acid. The use of cetyltrimethylammonium bromide precipitation sufficiently removed inhibitors to allow detection of as few as 10 PFU of poliovirus. Norwalk virus also could be detected after being seeded into oysters. This methodology may be useful for the detection of these and other shellfish-borne viral pathogens.

MeSH Terms

Animals Base Sequence Food Microbiology Hepatovirus/isolation & purification Molecular Sequence Data Norwalk virus/isolation & purification Ostreidae/microbiology Polymerase Chain Reaction

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Atmar R L

Department of Medicine, Baylor College of Medicine, Houston, Texas 77030.

Metcalf T G

Neill F H

Estes M K

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23 references, click to expand

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Article Info

Journal

Applied and environmental microbiology

Abbr.

Appl Environ Microbiol

ISSN

0099-2240

DOI

10.1128/aem.59.2.631-635.1993

Published

1993-02-00

Pages

631-5

Language

English

Region

United States

NLM ID

7605801

PMCID

PMC202159

Subset

Grants

NCRR NIH HHS · RR-00350 · United States

External Links

PubMed source DOI: 10.1128/aem.59.2.631-635.1993 PMC Full Text View journal details

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Detection of enteric viruses in oysters by using the polymerase chain reaction.

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