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PMID: 8381786 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The Ca2+-release channel/ryanodine receptor is localized in junctional and corbular sarcoplasmic reticulum in cardiac muscle.

The Journal of cell biology ·Vol. 120 ·No. 4 ·1993-02-00 ·Pages 969-80

Jorgensen AO, Shen AC, Arnold W, McPherson PS, Campbell KP

Abstract

The subcellular distribution of the Ca(2+)-release channel/ryanodine receptor in adult rat papillary myofibers has been determined by immunofluorescence and immunoelectron microscopical studies using affinity purified antibodies against the ryanodine receptor. The receptor is confined to the sarcoplasmic reticulum (SR) where it is localized to interior and peripheral junctional SR and the corbular SR, but it is absent from the network SR where the SR-Ca(2+)-ATPase and phospholamban are densely distributed. Immunofluorescence labeling of sheep Purkinje fibers show that the ryanodine receptor is confined to discrete foci while the SR-Ca(2+)-ATPase is distributed in a continuous network-like structure present at the periphery as well as throughout interior regions of these myofibers. Because Purkinje fibers lack T-tubules, these results indicate that the ryanodine receptor is localized not only to the peripheral junctional SR but also to corbular SR densely distributed in interfibrillar spaces of the I-band regions. We have previously identified both corbular SR and junctional SR in cardiac muscle as potential Ca(2+)-storage/Ca(2+)-release sites by demonstrating that the Ca2+ binding protein calsequestrin and calcium are very densely distributed in these two specialized domains of cardiac SR in situ. The results presented here provide strong evidence in support of the hypothesis that corbular SR is indeed a site of Ca(2+)-induced Ca2+ release via the ryanodine receptor during excitation contraction coupling in cardiac muscle. Furthermore, these results indicate that the function of the cardiac Ca(2+)-release channel/ryanodine receptor is not confined to junctional complexes between SR and the sarcolemma.

MeSH Terms
Animals Antibody Specificity Calcium/metabolism Calcium Channels/metabolism Fluorescent Antibody Technique Immunohistochemistry Muscle Contraction Myocardium/metabolism,ultrastructure Purkinje Cells/metabolism Rats Receptors, Cholinergic/immunology,metabolism Ryanodine Receptor Calcium Release Channel Sarcoplasmic Reticulum/immunology,metabolism Sheep
Chemicals
Calcium Channels Receptors, Cholinergic Ryanodine Receptor Calcium Release Channel Calcium
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Jorgensen A O
Department of Anatomy and Cell Biology, University of Toronto, Ontario, Canada.
Shen A C
Arnold W
McPherson P S
Campbell K P
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.120.4.969
Published
1993-02-00
Pages
969-80
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2200068
Subset
IM
Grants
NHLBI NIH HHS · HL39265 · United States
External Links
PubMed source DOI: 10.1083/jcb.120.4.969 PMC Full Text View journal details
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