Home LiteratureArticle Details
PMID: 8381783 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Autophosphorylation of nucleoside diphosphate kinase from Myxococcus xanthus.

Journal of bacteriology ·Vol. 175 ·No. 4 ·1993-02-00 ·Pages 1176-81

Muñoz-Dorado J, Almaula N, Inouye S, Inouye M

Abstract

The nucleoside diphosphate kinase (NDP kinase) from Myxococcus xanthus has been purified to homogeneity and crystallized (J. Munoz-Dorado, M. Inouye, and S. Inouye, J. Biol. Chem. 265:2702-2706, 1990). In the presence of ATP, the NDP kinase was autophosphorylated. Phosphoamino acid analysis was carried out after acid and base hydrolyses of phosphorylated NDP kinase. It was found that the protein was phosphorylated not only at a histidine residue but also at a serine residue. Replacement of histidine 117 with a glutamine residue completely abolished the autophosphorylation and nucleotide-binding activity of the NDP kinase. Since histidine 117 is the only histidine residue that is conserved in all known NDP kinases so far characterized, the results suggest that the phosphohistidine intermediate is formed at this residue during the transphosphorylation reaction from nucleoside triphosphates to nucleoside diphosphates. Preliminary mutational analysis of putative ATP-binding sites is also presented.

MeSH Terms
Adenosine Triphosphate/metabolism Amino Acid Sequence Base Sequence Hydrogen-Ion Concentration Molecular Sequence Data Mutagenesis, Site-Directed Myxococcus xanthus/enzymology Nucleoside-Diphosphate Kinase/chemistry,genetics,metabolism Nucleotides/metabolism Oligodeoxyribonucleotides/chemistry Phosphorylation Structure-Activity Relationship
Chemicals
Nucleotides Oligodeoxyribonucleotides Adenosine Triphosphate Nucleoside-Diphosphate Kinase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Muñoz-Dorado J
Department of Biochemistry, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Rutgers, Piscataway 08854.
Almaula N
Inouye S
Inouye M
References (30)
30 references, click to expand
  1. Identification of a phosphate-incorporating protein from bovine liver as nucleoside diphosphate kinase and isolation of 1-32P-phosphohistidine, 3-32P-phosphohistidine, and N-epsilon-32P-phospholysine from erythrocytic nucleoside diphosphate kinase, incubated with adenosine triphosphate-32P.
    J Biol Chem. 1968 Jul 25;243(14):3947-52 PMID: 5661717
  2. Some reactions of adenosine and inosine phosphates in animal tissues.
    Biochim Biophys Acta. 1953 Sep-Oct;12(1-2):172-80 PMID: 13115426
  3. Purification and properties of Bacillus subtilis nucleoside diphosphokinase.
    J Biol Chem. 1971 Sep 10;246(17):5365-72 PMID: 4999354
  4. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  5. Transforming gene product of Rous sarcoma virus phosphorylates tyrosine.
    Proc Natl Acad Sci U S A. 1980 Mar;77(3):1311-5 PMID: 6246487
  6. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  7. Structural details of the binding of guanosine diphosphate to elongation factor Tu from E. coli as studied by X-ray crystallography.
    EMBO J. 1985 Sep;4(9):2385-8 PMID: 3908095
  8. Three-dimensional structure of an oncogene protein: catalytic domain of human c-H-ras p21.
    Science. 1988 Feb 19;239(4842):888-93 PMID: 2448879
  9. Membrane-associated nucleoside diphosphate kinase from rat liver. Purification, characterization, and comparison with cytosolic enzyme.
    J Biol Chem. 1988 Apr 5;263(10):4647-53 PMID: 2832402
  10. The protein kinase family: conserved features and deduced phylogeny of the catalytic domains.
    Science. 1988 Jul 1;241(4861):42-52 PMID: 3291115
  11. Histidine phosphorylation and phosphoryl group transfer in bacterial chemotaxis.
    Nature. 1988 Nov 10;336(6195):139-43 PMID: 3185734
  12. Analysis of the lethal interaction between the prune and Killer of prune mutations of Drosophila.
    Genes Dev. 1988 Oct;2(10):1333-43 PMID: 2849580
  13. Acid and base hydrolysis of phosphoproteins bound to immobilon facilitates analysis of phosphoamino acids in gel-fractionated proteins.
    Anal Biochem. 1989 Jan;176(1):22-7 PMID: 2540676
  14. Structure of the guanine-nucleotide-binding domain of the Ha-ras oncogene product p21 in the triphosphate conformation.
    Nature. 1989 Sep 21;341(6239):209-14 PMID: 2476675
  15. Reduced Nm23/Awd protein in tumour metastasis and aberrant Drosophila development.
    Nature. 1989 Nov 9;342(6246):177-80 PMID: 2509941
  16. Nucleoside diphosphate kinase from Myxococcus xanthus. I. Cloning and sequencing of the gene.
    J Biol Chem. 1990 Feb 15;265(5):2702-6 PMID: 2154455
  17. Nucleoside diphosphate kinase from Myxococcus xanthus. II. Biochemical characterization.
    J Biol Chem. 1990 Feb 15;265(5):2707-12 PMID: 2154456
  18. Functional cloning of a nucleoside diphosphate kinase from Dictyostelium discoideum.
    J Biol Chem. 1990 Jun 15;265(17):10012-8 PMID: 2161830
  19. Dictyostelium nucleoside diphosphate kinase highly homologous to Nm23 and Awd proteins involved in mammalian tumor metastasis and Drosophila development.
    J Natl Cancer Inst. 1990 Jul 18;82(14):1199-202 PMID: 2163458
  20. Isolation and characterization of a cDNA clone encoding rat nucleoside diphosphate kinase.
    J Biol Chem. 1990 Sep 15;265(26):15744-9 PMID: 2168422
  21. A Drosophila gene that is homologous to a mammalian gene associated with tumor metastasis codes for a nucleoside diphosphate kinase.
    Cell. 1990 Nov 30;63(5):933-40 PMID: 2175255
  22. The GTPase superfamily: conserved structure and molecular mechanism.
    Nature. 1991 Jan 10;349(6305):117-27 PMID: 1898771
  23. Identification of a second human nm23 gene, nm23-H2.
    Cancer Res. 1991 Jan 1;51(1):445-9 PMID: 1988104
  24. Crystallization and preliminary X-ray diffraction studies of nucleoside diphosphate kinase from Dictyostelium discoideum.
    J Mol Biol. 1991 Jan 20;217(2):239-40 PMID: 1846924
  25. Nucleoside diphosphate kinase from human erythrocytes. Structural characterization of the two polypeptide chains responsible for heterogeneity of the hexameric enzyme.
    J Biol Chem. 1991 May 15;266(14):8784-9 PMID: 1851158
  26. Crystallization and preliminary X-ray diffraction analysis of oucleoside diphosphate kinase from Myxococcus xanthus.
    J Mol Biol. 1991 Jul 5;220(1):5-7 PMID: 1648623
  27. Nucleoside diphosphate kinase from Escherichia coli; its overproduction and sequence comparison with eukaryotic enzymes.
    Gene. 1991 Aug 30;105(1):31-6 PMID: 1657712
  28. Chemical properties and separation of phosphoamino acids by thin-layer chromatography and/or electrophoresis.
    Methods Enzymol. 1991;201:10-21 PMID: 1943759
  29. Transphosphorylation between nucleoside polyphosphates.
    Nature. 1953 Nov 28;172(4387):1008-9 PMID: 13111245
  30. Preparation of crystalline nucleoside diphosphate kinase from baker's yeast and identification of 1-[32P]phosphohistidine as the main phosphorylated product of an alkaline hydrolysate of enzyme incubated with adenosine [32P]triphosphate.
    Eur J Biochem. 1969 Jul;9(4):451-5 PMID: 5806496
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.4.1176-1181.1993
Published
1993-02-00
Pages
1176-81
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC193035
Subset
IM
Grants
NIGMS NIH HHS · GM26843 · United States
External Links
PubMed source DOI: 10.1128/jb.175.4.1176-1181.1993 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2