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PMID: 8380148 Published · ppublish English Journal Article

Characterization of Tn1546, a Tn3-related transposon conferring glycopeptide resistance by synthesis of depsipeptide peptidoglycan precursors in Enterococcus faecium BM4147.

Journal of bacteriology ·Vol. 175 ·No. 1 ·1993-01-00 ·Pages 117-27

Arthur M, Molinas C, Depardieu F, Courvalin P

Abstract

Sequence determination of the flanking regions of the vancomycin resistance van gene cluster carried by pIP816 in Enterococcus faecium BM4147 revealed similarity to transposons of the Tn3 family. Imperfect inverted repeats (36 of 38 bp) delineated a 10,851-bp element designated Tn1546. The 4-kb region located upstream from the vanR gene contained two open reading frames (ORF) transcribed in opposite directions. The deduced amino acid sequence of ORF1 (988 residues) displayed, respectively, 56 and 42% identity to those of the transposases of Tn4430 from Bacillus thuringiensis and of Tn917 from Enterococcus faecalis. The product of ORF2 (191 residues) was related to the resolvase of Tn917 (33% amino acid identity) and to the Res protein (48%) of plasmid pIP404 from Clostridium perfringens. Tn1546 transposed consecutively in Escherichia coli from plasmid pUC18 into pOX38 and from pOX38 into various sites of pBR329. Transposition was replicative, led to the formation of cointegrates, and produced a 5-bp duplication at the target site. Southern hybridization and DNA amplification revealed the presence of Tn1546-related elements in enterococci highly resistant to glycopeptides. Analysis of sequences surrounding these elements indicated that transposition plays a role in dissemination of the van gene cluster among replicons of human clinical isolates of E. faecium.

MeSH Terms
Alanine/analogs & derivatives,metabolism Amino Acid Sequence Anti-Bacterial Agents/pharmacology Base Sequence Chromosome Inversion Conjugation, Genetic DNA Transposable Elements/genetics Drug Resistance, Microbial/genetics Enterococcus faecium/genetics Genes, Bacterial/genetics Gram-Positive Bacterial Infections/genetics Lactates/metabolism Molecular Sequence Data Mutagenesis, Insertional/genetics Nucleotidyltransferases/genetics Open Reading Frames/genetics Plasmids/genetics Repetitive Sequences, Nucleic Acid/genetics Replicon/genetics Sequence Homology, Amino Acid Sequence Homology, Nucleic Acid Transposases Vancomycin/pharmacology
Chemicals
Anti-Bacterial Agents DNA Transposable Elements Lactates alanyllactate Vancomycin Nucleotidyltransferases Transposases Alanine
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Arthur M
Unité des Agents Antibactériens, Institut Pasteur, Paris, France.
Molinas C
Depardieu F
Courvalin P
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.1.117-127.1993
Published
1993-01-00
Pages
117-27
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC196104
Subset
IM
Databases
GENBANK
M97297
External Links
PubMed source DOI: 10.1128/jb.175.1.117-127.1993 PMC Full Text View journal details
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