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PMID: 8376346 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Bacillus subtilis alkA gene encoding inducible 3-methyladenine DNA glycosylase is adjacent to the ada operon.

Journal of bacteriology ·Vol. 175 ·No. 18 ·1993-09-00 ·Pages 6010-7

Morohoshi F, Hayashi K, Munkata N

Abstract

In Bacillus subtilis, the adaptive response to DNA alkylation depends on the ada operon, which consists of the adaA and adaB genes, which encode methylphosphotriester DNA methyltransferase (AdaA protein) and O6-methylguanine DNA methyltransferase (AdaB protein), respectively. A structural gene (alkA) that encodes 3-methyladenine DNA glycosylase was found upstream of the ada operon, but in the opposite orientation. This cluster of genes was mapped at about 235 kb from the SfiI recognition site near the origin of replication in the physical map of the B. subtilis chromosome. Disruption of the alkA gene sensitized cells to N-propyl-N'-nitro-N-nitrosoguanidine, while its overproduction rendered cells highly resistant to N-propyl-N'-nitro-N-nitrosoguanidine, indicating that lethal DNA damage produced by bulky alkylating agents was effectively counteracted by AlkA glycosylase. Transcription of the alkA gene was induced by treating adaA+ cells with methylating agents concurrent with transcription of the ada operon. This was accomplished by using methylated AdaA protein bound to a 30-bp segment in the middle of the 100-bp sequence between the transcriptional start sites of the alkA gene and ada operon. Thus, in this organism, the adaptive response to DNA alkylation is achieved by autologous activation of a divergent regulon composed of the genes for a DNA glycosylase and two species of DNA alkyltransferase.

Related Genes
adaA adaB alkA
MeSH Terms
Alkylating Agents/pharmacology Alkylation Amino Acid Sequence Bacillus subtilis/enzymology,genetics Bacterial Proteins/genetics Base Sequence Cloning, Molecular DNA Glycosylases DNA Repair DNA, Bacterial Deoxyribonuclease I Enzyme Induction Escherichia coli Escherichia coli Proteins Methyltransferases/genetics Molecular Sequence Data N-Glycosyl Hydrolases/biosynthesis,genetics O(6)-Methylguanine-DNA Methyltransferase Open Reading Frames Operon Restriction Mapping Sequence Homology, Amino Acid Transcription Factors
Chemicals
Alkylating Agents Bacterial Proteins DNA, Bacterial Escherichia coli Proteins Transcription Factors Methyltransferases methylphosphotriester methyltransferase Ada protein, E coli O(6)-Methylguanine-DNA Methyltransferase Deoxyribonuclease I 3-methyladenine-DNA glycosylase DNA Glycosylases N-Glycosyl Hydrolases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Morohoshi F
Radiobiology Division, National Cancer Center Research Institute, Tokyo, Japan.
Hayashi K
Munkata N
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38 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.18.6010-6017.1993
Published
1993-09-00
Pages
6010-7
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206683
Subset
IM
Databases
GENBANK
D14465
External Links
PubMed source DOI: 10.1128/jb.175.18.6010-6017.1993 PMC Full Text View journal details
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