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PMID: 8355657 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Potentially rapid walking in cellular regulatory networks using the gene-gene interference method in yeast.

Molecular & general genetics : MGG ·Vol. 240 ·No. 2 ·1993-08-00 ·Pages 245-57

Daniel J

Abstract

The recognition that cellular regulatory circuitry is composed of antagonistic elements has made possible a new approach to the selection of regulatory genes, called the gene-gene interference method. The method was used to identify and isolate genes possibly related in an antagonistic way to protein kinase A. Two such genes were recovered: ART1 encodes a potential regulator of cytokinesis and KAI1 appears to be involved in the Start control. The principles of the gene-gene interference method are discussed, as well as its possible general use for 'walking' within the cellular regulatory networks of eukaryotes.

Related Genes
ADE2 ART1 KAI1
MeSH Terms
Amino Acid Sequence Antigens, CD Base Sequence Calcium-Binding Proteins Cell Division/genetics Chromosome Walking/methods Cloning, Molecular DNA, Fungal DNA-Binding Proteins Fungal Proteins/genetics Gene Expression Regulation, Fungal Genes, Fungal Genes, Regulator Genetic Vectors Intracellular Signaling Peptides and Proteins Kangai-1 Protein Membrane Glycoproteins Membrane Proteins Molecular Sequence Data Plasmids Protein Kinase Inhibitors Protein Kinases/genetics Proto-Oncogene Proteins Saccharomyces cerevisiae/enzymology,genetics,growth & development Saccharomyces cerevisiae Proteins Sequence Homology, Amino Acid Transcription Factors Transformation, Genetic
Chemicals
Antigens, CD Calcium-Binding Proteins DNA, Fungal DNA-Binding Proteins Fungal Proteins Intracellular Signaling Peptides and Proteins Kangai-1 Protein MID2 protein, S cerevisiae Membrane Glycoproteins Membrane Proteins Protein Kinase Inhibitors Proto-Oncogene Proteins SWI4 protein, S cerevisiae Saccharomyces cerevisiae Proteins Transcription Factors Protein Kinases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Daniel J
Department of Biochemistry, Weizmann Institute of Science, Rehovot, Israel.
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00277063
Published
1993-08-00
Pages
245-57
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00277063
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