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PMID: 8351200 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Patch-clamp recordings from the soma and dendrites of neurons in brain slices using infrared video microscopy.

Pflugers Archiv : European journal of physiology ·Vol. 423 ·No. 5-6 ·1993-06-00 ·Pages 511-8

Stuart GJ, Dodt HU, Sakmann B

Abstract

A description is given of the implementation of infrared differential interference contrast (IR-DIC) video microscopy to an upright compound microscope. Using the improved resolution offered by IR-DIC a procedure is described for making patch-pipette recordings from visually identified neuronal somata and dendrites in brain slices. As an example of the application of this technique to electrophysiological recordings from small neuronal processes in brain slices we describe whole-cell current-clamp and cell-attached and excised patch-clamp recordings from the apical dendrites of layer V pyramidal neurons in slices of rat neocortex.

MeSH Terms
Animals Brain/cytology,physiology Cerebral Cortex/cytology,physiology Dendrites/physiology,ultrastructure Electrophysiology In Vitro Techniques Infrared Rays Neurons/physiology,ultrastructure Pyramidal Tracts/cytology,physiology Rats Video Recording
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Stuart G J
Max-Planck-Institut für medizinische Forschung, Abteilung Zellphysiologie, Heidelberg, Germany.
Dodt H U
Sakmann B
References (11)
11 references, click to expand
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Article Info
Journal
Pflugers Archiv : European journal of physiology
Abbr.
Pflugers Arch
ISSN
0031-6768
DOI
10.1007/BF00374949
Published
1993-06-00
Pages
511-8
Language
English
Region
Germany
NLM ID
0154720
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00374949 View journal details
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