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PMID: 8341699 Published · ppublish English Case Reports Journal Article Research Support, U.S. Gov't, P.H.S.

Rare scleroderma autoantibodies to the U11 small nuclear ribonucleoprotein and to the trimethylguanosine cap of U small nuclear RNAs.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 14 ·1993-07-15 ·Pages 6781-5

Gilliam AC, Steitz JA

Abstract

We have identified a scleroderma serum (Ru) with a previously undescribed specificity to protein components of the U11 small nuclear ribonucleoprotein particle (snRNP), a low-abundance member of the Sm class of U RNPs. The U11 RNP can be specifically immunoprecipitated from sonicated HeLa cells with Ru serum. In nuclear extracts, a fraction of the U11 particle is found complexed to the U12 RNP, an even lower abundance Sm snRNP. In glycerol gradient fractions, Ru serum identifies a 65-kDa protein that cosediments with the U11-U12 complex and is shifted upon targeted degradation of the U12 RNA. The 65-kDa protein therefore appears to be a component of the U11-U12 snRNP complex, whereas another Ru-reactive (140 kDa) protein may be associated with the free U11 RNP. The Ru serum also contains autoantibodies directed against the trimethylguanosine cap of U RNAs. This rare specificity has been described previously in only three other scleroderma patients.

MeSH Terms
Adult Antibodies, Antinuclear/immunology Antibody Specificity Female Guanosine/analogs & derivatives,immunology Humans RNA Caps/immunology RNA, Small Nuclear/immunology Ribonucleoproteins, Small Nuclear/chemistry,immunology Scleroderma, Systemic/immunology Sequence Analysis, RNA
Chemicals
Antibodies, Antinuclear RNA Caps RNA, Small Nuclear Ribonucleoproteins, Small Nuclear Guanosine N(2),N(2),7-trimethylguanosine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Gilliam A C
Department of Dermatology, Yale University School of Medicine, New Haven, CT.
Steitz J A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.14.6781
Published
1993-07-15
Pages
6781-5
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47016
Subset
IM
Grants
NIAMS NIH HHS · 5-T32-AR07016 · United States
NIGMS NIH HHS · 5RO1-GM26154 · United States
External Links
PubMed source DOI: 10.1073/pnas.90.14.6781 PMC Full Text View journal details
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