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PMID: 8335000 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A 102 kDa subunit of a Golgi-associated particle has homology to beta subunits of trimeric G proteins.

The EMBO journal ·Vol. 12 ·No. 7 ·1993-07-00 ·Pages 2847-53

Harrison-Lavoie KJ, Lewis VA, Hynes GM, Collison KS, Nutland E, Willison KR

Abstract

We have identified a 102 kDa protein, p102, which is found on the cytoplasmic face of Golgi membranes, exocytic transport vesicles and in the cytosol. A monoclonal antibody that cross-reacts with p102 is able to immunoprecipitate a 500-600 kDa protein complex containing p102 and additional subunits. The composition of this p102-containing protein complex resembles that of the Golgi coatomer complex, which constitutes the coat of non-clathrin coated vesicles. One of the subunits of the p102 complex reacts with a monoclonal antibody that detects beta-COP, a subunit of the Golgi coatomer complex. Like beta-COP, p102 exists in a brefeldin A-sensitive association with Golgi membranes. The sequence of p102 contains an N-terminal domain composed of six repeats which are similar to those found in the beta subunit of trimeric G proteins and other regulatory proteins. We suggest that p102 may be involved in regulating membrane traffic in the constitutive exocytic pathway.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal/immunology Blotting, Western Brefeldin A Cloning, Molecular Coatomer Protein Cross Reactions Cyclopentanes/pharmacology DNA GTP-Binding Proteins/chemistry Golgi Apparatus/chemistry HeLa Cells Humans Intracellular Membranes/chemistry Membrane Proteins/chemistry,immunology Mice Molecular Sequence Data Sequence Homology, Amino Acid Transducin/chemistry
Chemicals
Antibodies, Monoclonal Coatomer Protein Cyclopentanes Membrane Proteins Brefeldin A DNA GTP-Binding Proteins Transducin
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Harrison-Lavoie K J
Institute of Cancer Research, Chester Beatty Laboratories, London, UK.
Lewis V A
Hynes G M
Collison K S
Nutland E
Willison K R
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1993-07-00
Pages
2847-53
Language
English
Region
England
NLM ID
8208664
PMCID
PMC413536
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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