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PMID: 8317549 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Detection of clonal B cell populations in paraffin-embedded tissues by polymerase chain reaction.

The American journal of pathology ·Vol. 143 ·No. 1 ·1993-07-00 ·Pages 230-9

Küppers R, Zhao M, Rajewsky K, Hansmann ML

Abstract

A method was established to detect clonal B cell populations in frozen and paraffin-embedded tissues. The method is based on the polymerase chain reaction amplification of rearranged VH and V kappa genes using V gene family-specific primers. Monoclonal B cell populations could be detected in peripheral blood lymphocyte DNA in all 16 cases of B-CLL and immunocytoma investigated and in 8 of 10 cases of B cell non-Hodgkin's lymphoma using frozen and paraffin sections. The amplification of V kappa rearrangements in addition to the VH amplification is a useful tool to verify the results of the heavy chain rearrangement and to detect proliferation of a B cell clone in cases in which no VH product was obtained. In spite of the degradation of DNA in paraffin-embedded tissues, we were able to find amplified polymerase chain reaction products of about 350 bp length in 8 of 10 cases analyzed. The method presented here may be helpful in routine diagnosis of B cell non-Hodgkin's lymphoma using frozen or paraffin-embedded specimens.

Related Genes
D<down>H</down> J<down>&kgr </down> V<down>&kgr </down> V<down>H</down>
MeSH Terms
B-Lymphocyte Subsets/cytology Base Sequence Blotting, Southern Clone Cells/cytology Electrophoresis, Agar Gel Frozen Sections Gene Amplification Gene Rearrangement, B-Lymphocyte, Heavy Chain Gene Rearrangement, B-Lymphocyte, Light Chain Humans Immunoglobulin Heavy Chains/genetics Immunoglobulin Light Chains/genetics Leukemia, B-Cell/genetics Molecular Sequence Data Paraffin Embedding Polymerase Chain Reaction
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin Light Chains
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Küppers R
I. Medizinische Universitätsklinik Köln, Germany.
Zhao M
Rajewsky K
Hansmann M L
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31 references, click to expand
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1993-07-00
Pages
230-9
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1886961
Subset
IM
External Links
PubMed source PMC Full Text View journal details
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