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PMID: 3141924 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Physical linkage of a human immunoglobulin heavy chain variable region gene segment to diversity and joining region elements.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 85 ·No. 21 ·1988-11-00 ·Pages 8196-200

Schroeder HW, Walter MA, Hofker MH, Ebens A, Willems van Dijk K, Liao LC, Cox DW, Milner EC, Perlmutter RM

Abstract

Antibody genes are assembled from a series of germ-line gene segments that are juxtaposed during the maturation of B lymphocytes. Although diversification of the adult antibody repertoire results in large part from the combinatorial joining of these gene segments, a restricted set of antibody heavy chain variable (VH), diversity (DH), and joining (JH) region gene segments appears preferentially in the human fetal repertoire. We report here that one of these early-expressed VH elements (termed VH6) is the most 3' VH gene segment, positioned 77 kilobases on the 5' side of the JH locus and immediately adjacent to a set of previously described DH sequences. In addition to providing a physical map linking human VH, DH, and JH elements, these results support the view that the programmed development of the antibody VH repertoire is determined in part by the chromosomal position of these gene segments.

MeSH Terms
B-Lymphocytes/analysis Base Sequence Cosmids Genes, Immunoglobulin Genetic Linkage Humans Immunoglobulin Heavy Chains/genetics Immunoglobulin Variable Region/genetics Molecular Sequence Data
Chemicals
Immunoglobulin Heavy Chains Immunoglobulin Variable Region
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Schroeder H W
Howard Hughes Medical Institute, University of Washington, Seattle 98195.
Walter M A
Hofker M H
Ebens A
Willems van Dijk K
Liao L C
Cox D W
Milner E C
Perlmutter R M
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.85.21.8196
Published
1988-11-00
Pages
8196-200
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC282394
Subset
IM
Databases
GENBANK
J04097
External Links
PubMed source DOI: 10.1073/pnas.85.21.8196 PMC Full Text View journal details
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