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PMID: 8317544 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Sequential expression of transforming growth factors alpha and beta 1 by eosinophils during cutaneous wound healing in the hamster.

The American journal of pathology ·Vol. 143 ·No. 1 ·1993-07-00 ·Pages 130-42

Wong DT, Donoff RB, Yang J, Song BZ, Matossian K, Nagura N, Elovic A, McBride J, Gallagher G, Todd R

Abstract

Transforming growth factor-alpha (TGF-alpha) and TGF-beta 1 have been proposed as important regulators of processes critical to successful wound healing. Although various cells present in wounds represent potential sources of either TGF-alpha and/or TGF-beta, including macrophages, neutrophils, keratinocytes, fibroblasts, and endothelial cells, we recently identified eosinophils as an additional potential source of these cytokines. We therefore used in situ hybridization and immunohistochemistry to determine whether eosinophils represent significant sources of TGF-alpha and/or TGF-beta 1 in skin wounds in the hamster. We found that these wounds developed a prominent infiltration of eosinophils, and that eosinophils were a cellular source of both TGF-alpha and TGF-beta 1 mRNAs. TGF-alpha and TGF-beta 1 proteins were detectable both within eosinophils and extracellularly. Moreover, there was a sequential pattern of TGF-alpha and TGF-beta 1 expression by infiltrating eosinophils, with the onset of eosinophil-associated TGF-alpha expression preceding that of TGF-beta 1. This sequential pattern of TGF expression suggests that eosinophils may help to regulate critical biological processes during wound healing.

MeSH Terms
Amino Acid Sequence Animals Base Sequence Cloning, Molecular Cricetinae Eosinophils/chemistry Immunohistochemistry In Situ Hybridization Male Molecular Sequence Data Phenotype RNA, Messenger/analysis,genetics Skin/injuries Time Factors Transforming Growth Factor alpha/blood,genetics Transforming Growth Factor beta/blood,genetics Wound Healing/physiology
Chemicals
RNA, Messenger Transforming Growth Factor alpha Transforming Growth Factor beta
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Wong D T
Department of Oral Medicine, Harvard School of Dental Medicine, Boston, MA 02115.
Donoff R B
Yang J
Song B Z
Matossian K
Nagura N
Elovic A
McBride J
Gallagher G
Todd R
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Article Info
Journal
The American journal of pathology
Abbr.
Am J Pathol
ISSN
0002-9440
Published
1993-07-00
Pages
130-42
Language
English
Region
United States
NLM ID
0370502
PMCID
PMC1886938
Subset
IM
Grants
NIAID NIH HHS · AI-22674 · United States
NIAID NIH HHS · AI-23990 · United States
NIDCR NIH HHS · DE-08680 · United States
Databases
GENBANK
L15427, L15428, L20160, L20161, L20162, L20163, L20164, L20165, L20166, X60296
External Links
PubMed source PMC Full Text View journal details
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