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PMID: 8313912 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A role for calnexin (IP90) in the assembly of class II MHC molecules.

The EMBO journal ·Vol. 13 ·No. 3 ·1994-02-01 ·Pages 675-82

Anderson KS, Cresswell P

Abstract

Major histocompatibility complex (MHC) class II antigens consist of alpha and beta chains that associate intracellularly with the invariant (I) chain. The HLA-DR alpha beta I complex assembles in the endoplasmic reticulum (ER) into a nonameric structure via progressive addition of three alpha beta dimers to a core invariant chain trimer. We have examined intracellular association of alpha beta I complexes with the resident ER protein calnexin. Calnexin associates rapidly (within 3 min) with newly synthesized alpha, beta and I chains, and remains associated with the assembling alpha beta I complex until the final alpha beta dimer is added, forming the complete nonamer. Dissociation of calnexin parallels egress of alpha beta I from the ER. These results suggest that calnexin retains and stabilizes both free class II subunits and partially assembled class II-I chain complexes until assembly of the nonamer is complete.

MeSH Terms
Calcium-Binding Proteins/metabolism Calnexin Electrophoresis, Gel, Two-Dimensional HeLa Cells Histocompatibility Antigens Class II/metabolism Humans
Chemicals
Calcium-Binding Proteins Histocompatibility Antigens Class II Calnexin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Anderson K S
Section of Immunobiology, Howard Hughes Medical Institute, Yale University School of Medicine, New Haven, CT 06510.
Cresswell P
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-02-01
Pages
675-82
Language
English
Region
England
NLM ID
8208664
PMCID
PMC394858
Subset
IM
Grants
NIAID NIH HHS · AI-15775 · United States
External Links
PubMed source PMC Full Text View journal details
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