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PMID: 8300517 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

nodZ, a unique host-specific nodulation gene, is involved in the fucosylation of the lipooligosaccharide nodulation signal of Bradyrhizobium japonicum.

Journal of bacteriology ·Vol. 176 ·No. 3 ·1994-02-00 ·Pages 620-33

Stacey G, Luka S, Sanjuan J, Banfalvi Z, Nieuwkoop AJ, Chun JY, Forsberg LS, Carlson R

Abstract

The nodulation genes of rhizobia are regulated by the nodD gene product in response to host-produced flavonoids and appear to encode enzymes involved in the production of a lipo-chitose signal molecule required for infection and nodule formation. We have identified the nodZ gene of Bradyrhizobium japonicum, whose product is required for the addition of a 2-O-methylfucose residue to the terminal reducing N-acetylglucosamine of the nodulation signal. This substitution is essential for the biological activity of this molecule. Mutations in nodZ result in defective nodulation of siratro. Surprisingly, although nodZ clearly codes for nodulation function, it is not regulated by NodD and, indeed, shows elevated expression in planta. Therefore, nodZ represents a unique nodulation gene that is not under the control of NodD and yet is essential for the synthesis of an active nodulation signal.

Related Genes
hsn nodZ
MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular Fucosyltransferases/metabolism Gene Expression Genes, Bacterial Lipopolysaccharides/metabolism Molecular Sequence Data Mutagenesis, Site-Directed Plants/microbiology Promoter Regions, Genetic RNA, Messenger/genetics Restriction Mapping Rhizobiaceae/genetics,metabolism Transcription, Genetic
Chemicals
Lipopolysaccharides RNA, Messenger Fucosyltransferases
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Stacey G
Department of Microbiology and Graduate Program of Ecology, University of Tennessee, Knoxville 37996-0845.
Luka S
Sanjuan J
Banfalvi Z
Nieuwkoop A J
Chun J Y
Forsberg L S
Carlson R
References (45)
45 references, click to expand
  1. The pleiotropic nature of symbiotic regulatory mutants: Bradyrhizobium japonicum nifA gene is involved in control of nif gene expression and formation of determinate symbiosis.
    EMBO J. 1986 Jun;5(6):1165-73 PMID: 15966104
  2. Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti.
    Proc Natl Acad Sci U S A. 1980 Dec;77(12):7347-51 PMID: 7012838
  3. Signaling and host range variation in nodulation.
    Annu Rev Microbiol. 1992;46:497-531 PMID: 1444265
  4. Host-specific regulation of nodulation genes in Rhizobium is mediated by a plant-signal, interacting with the nodD gene product.
    EMBO J. 1987 Apr;6(4):841-8 PMID: 16453758
  5. nodO, a new nod gene of the Rhizobium leguminosarum biovar viciae sym plasmid pRL1JI, encodes a secreted protein.
    J Bacteriol. 1989 Dec;171(12):6764-70 PMID: 2687250
  6. Identification of Bradyrhizobium nod genes involved in host-specific nodulation.
    J Bacteriol. 1989 Jun;171(6):3324-30 PMID: 2542223
  7. Identification of a nodD-dependent locus in the Rhizobium strain NGR234 activated by phenolic factors secreted by soybeans and other legumes.
    Mol Plant Microbe Interact. 1988 Apr;1(4):161-8 PMID: 2980201
  8. Host recognition in the Rhizobium-soybean symbiosis: detection of a protein factor in soybean root exudate which is involved in the nodulation process.
    Plant Physiol. 1984 Jan;74(1):84-9 PMID: 16663392
  9. Direct selection for P1-sensitive mutants of enteric bacteria.
    J Bacteriol. 1974 Jun;118(3):810-4 PMID: 4598005
  10. Evidence for two functional gal promoters in intact Escherichia coli cells.
    J Biol Chem. 1981 Nov 25;256(22):11905-10 PMID: 6271763
  11. Isolation and characterization of the DNA region encoding nodulation functions in Bradyrhizobium japonicum.
    J Bacteriol. 1985 Dec;164(3):1301-8 PMID: 2999080
  12. Bacteriophage T4 late promoters: mapping 5' ends of T4 gene 23 mRNAs.
    EMBO J. 1982;1(1):107-14 PMID: 6201352
  13. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  14. Analysis of Rhizobium meliloti nodulation mutant WL131: novel insertion sequence ISRm3 in nodG and altered nodH protein product.
    J Bacteriol. 1991 May;173(10):3060-5 PMID: 1850728
  15. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity.
    Anal Biochem. 1983 Jul 1;132(1):6-13 PMID: 6312838
  16. Secretion of the Rhizobium leguminosarum nodulation protein NodO by haemolysin-type systems.
    Mol Microbiol. 1992 Jan;6(2):231-8 PMID: 1545707
  17. A Tn3 lacZ transposon for the random generation of beta-galactosidase gene fusions: application to the analysis of gene expression in Agrobacterium.
    EMBO J. 1985 Apr;4(4):891-8 PMID: 2990912
  18. Detection and separation of Rhizobium and Bradyrhizobium Nod metabolites using thin-layer chromatography.
    Mol Plant Microbe Interact. 1992 Jan-Feb;5(1):72-80 PMID: 1600238
  19. DNA hybridization probe for the Pseudomonas fluorescens group.
    Appl Environ Microbiol. 1986 Nov;52(5):1190-4 PMID: 3098169
  20. Heme biosynthesis in Rhizobium. Identification of a cloned gene coding for delta-aminolevulinic acid synthetase from Rhizobium meliloti.
    J Biol Chem. 1982 Aug 10;257(15):8724-30 PMID: 7096330
  21. Broad-host-range Rhizobium species strain NGR234 secretes a family of carbamoylated, and fucosylated, nodulation signals that are O-acetylated or sulphated.
    Mol Microbiol. 1992 Dec;6(23):3575-84 PMID: 1474899
  22. Role of the Rhizobium meliloti nodF and nodE genes in the biosynthesis of lipo-oligosaccharidic nodulation factors.
    J Biol Chem. 1993 Sep 25;268(27):20134-42 PMID: 8376372
  23. Rhizobium-legume nodulation: life together in the underground.
    Cell. 1989 Jan 27;56(2):203-14 PMID: 2643474
  24. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  25. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  26. Transposon-induced symbiotic mutants of Bradyrhizobium japonicum: isolation of two gene regions essential for nodulation.
    Mol Gen Genet. 1987 Apr;207(1):15-23 PMID: 3037278
  27. A locus encoding host range is linked to the common nodulation genes of Bradyrhizobium japonicum.
    J Bacteriol. 1987 Jun;169(6):2631-8 PMID: 3584066
  28. Simple method for identification of plasmid-coded proteins.
    J Bacteriol. 1979 Jan;137(1):692-3 PMID: 368040
  29. Bradyrhizobium japonicum nodD1 can be specifically induced by soybean flavonoids that do not induce the nodYABCSUIJ operon.
    J Biol Chem. 1992 Jan 5;267(1):310-8 PMID: 1730597
  30. Rhizobial lipo-oligosaccharides: answers and questions.
    Plant Mol Biol. 1992 Dec;20(5):977-86 PMID: 1463833
  31. "ATG vectors' for regulated high-level expression of cloned genes in Escherichia coli.
    Gene. 1985;40(2-3):183-90 PMID: 3007288
  32. Lipo-oligosaccharide nodulation factors: a minireview new class of signaling molecules mediating recognition and morphogenesis.
    Cell. 1993 Sep 24;74(6):951-4 PMID: 8402884
  33. Isolation of the Rhizobium leguminosarum NodF nodulation protein: NodF carries a 4'-phosphopantetheine prosthetic group.
    J Bacteriol. 1991 May;173(9):2872-8 PMID: 2019559
  34. R factor transfer in Rhizobium leguminosarum.
    J Gen Microbiol. 1974 Sep;84(1):188-98 PMID: 4612098
  35. A 2-O-methylfucose moiety is present in the lipo-oligosaccharide nodulation signal of Bradyrhizobium japonicum.
    Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8789-93 PMID: 1528893
  36. Rhizobium japonicum derivatives differing in nitrogen-fixing efficiency and carbohydrate utilization.
    Appl Environ Microbiol. 1976 Oct;32(4):511-9 PMID: 988784
  37. Regulation of nod gene expression in Bradyrhizobium japonicum.
    Mol Gen Genet. 1988 Nov;214(3):420-4 PMID: 3146016
  38. Molecular basis of symbiotic host specificity in Rhizobium meliloti: nodH and nodPQ genes encode the sulfation of lipo-oligosaccharide signals.
    Cell. 1991 Dec 20;67(6):1131-43 PMID: 1760841
  39. Use of bacteriophage P1 as a vector for Tn5 insertion mutagenesis.
    Appl Environ Microbiol. 1984 Feb;47(2):436-8 PMID: 6324677
  40. The structures and biological activities of the lipo-oligosaccharide nodulation signals produced by type I and II strains of Bradyrhizobium japonicum.
    J Biol Chem. 1993 Aug 25;268(24):18372-81 PMID: 8349712
  41. The Rhizobium nodulation gene nodO encodes a Ca2(+)-binding protein that is exported without N-terminal cleavage and is homologous to haemolysin and related proteins.
    EMBO J. 1990 Feb;9(2):349-54 PMID: 2303029
  42. Production of root hair deformation factors by Rhizobium meliloti nodulation genes in Escherichia coli: HsnD (NodH) is involved in the plant host-specific modification of the NodABC factor.
    Plant Mol Biol. 1989 Jul;13(1):1-12 PMID: 2562755
  43. Rhizobium--plant signal exchange.
    Nature. 1992 Jun 25;357(6380):655-60 PMID: 1614514
  44. ATP sulphurylase activity of the nodP and nodQ gene products of Rhizobium meliloti.
    Nature. 1990 Dec 13;348(6302):644-7 PMID: 2250719
  45. A general method for site-directed mutagenesis in prokaryotes.
    Nature. 1981 Jan 1;289(5793):85-8 PMID: 6256652
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.176.3.620-633.1994
Published
1994-02-00
Pages
620-33
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC205098
Subset
IM
Grants
NIGMS NIH HHS · GM39583 · United States
Databases
GENBANK
L22756
External Links
PubMed source DOI: 10.1128/jb.176.3.620-633.1994 PMC Full Text View journal details
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