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PMID: 2542223 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Identification of Bradyrhizobium nod genes involved in host-specific nodulation.

Journal of bacteriology ·Vol. 171 ·No. 6 ·1989-06-00 ·Pages 3324-30

Deshmane N, Stacey G

Abstract

Three loci important for soybean nodulation by Bradyrhizobium japonicum were delimited by Tn5 mutagenesis on a 5.3-kilobase EcoRI fragment adjacent to the nodABC genes. Results of hybridization studies suggested that this region is conserved in Bradyrhizobium species but absent in all Rhizobium species. lacZ translational fusions of two of the loci contained in this region were found to be inducible by host-produced flavonoid chemicals via a mechanism requiring a functional nodD gene product. A mutation in one of the loci was found to result in an alteration of the host range of B. japonicum. This mutation appears to block nodulation at the step at which plant root cortical cell division is induced.

MeSH Terms
Blotting, Southern DNA Mutational Analysis DNA Transposable Elements DNA, Bacterial/genetics Gene Expression Regulation Genes, Bacterial Restriction Mapping Rhizobiaceae/genetics Rhizobium/genetics Soybeans/microbiology Symbiosis
Chemicals
DNA Transposable Elements DNA, Bacterial
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Deshmane N
Department of Microbiology, University of Tennessee, Knoxville 37996-0845.
Stacey G
References (29)
29 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.171.6.3324-3330.1989
Published
1989-06-00
Pages
3324-30
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC210053
Subset
IM
Grants
NIGMS NIH HHS · GM 33494-04 · United States
NIGMS NIH HHS · GM 40183-01 · United States
External Links
PubMed source DOI: 10.1128/jb.171.6.3324-3330.1989 PMC Full Text View journal details
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