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PMID: 8265347 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structure of the gene encoding the 14.5 kDa subunit of human RNA polymerase II.

Nucleic acids research ·Vol. 21 ·No. 23 ·1993-11-25 ·Pages 5345-50

Acker J, Wintzerith M, Vigneron M, Kedinger C

Abstract

The structure of the gene encoding the 14.5 kDa subunit of the human RNA polymerase II (or B) has been elucidated. The gene consists of six exons, ranging from 52 to over 101 bp, interspaced with five introns ranging from 84 to 246 bp. It is transcribed into three major RNA species, present at low abundance in exponentially growing HeLa cells. The corresponding messenger RNAs contain the same open reading frame encoding a 125 amino acid residue protein, with a calculated molecular weight of 14,523 Da. This protein (named hRPB14.5) shares strong homologies with the homologous polymerase subunits encoded by the Drosophila (RpII15) and yeast (RPB9) genes. Cysteines characteristic of two zinc fingers are conserved in all three corresponding sequences and, like the yeast protein, the hRPB14.5 subunit exhibits zinc-binding activity.

Related Genes
RPB9 RpII15 hRPB14.5
MeSH Terms
Amino Acid Sequence Animals Base Sequence DNA, Complementary/genetics Drosophila Proteins Drosophila melanogaster/genetics Genes HeLa Cells Humans Molecular Sequence Data Nerve Tissue Proteins/genetics RNA Polymerase II/genetics RNA-Binding Proteins/genetics Saccharomyces cerevisiae/genetics Sequence Alignment Sequence Homology, Amino Acid Transcription, Genetic
Chemicals
DNA, Complementary Drosophila Proteins Nerve Tissue Proteins RNA-Binding Proteins Rbp9 protein, Drosophila RNA Polymerase II
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Acker J
Laboratoire de Génétique Moléculaire des Eucaryotes (CNRS), Unité 184 de Biologie Moléculaire et de Génie Génétique (INSERM), Institut de Chimie Biologique, Strasbourg, France.
Wintzerith M
Vigneron M
Kedinger C
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/21.23.5345
Published
1993-11-25
Pages
5345-50
Language
English
Region
England
NLM ID
0411011
PMCID
PMC310569
Subset
IM
Databases
GENBANK
Z23102
External Links
PubMed source DOI: 10.1093/nar/21.23.5345 PMC Full Text View journal details
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