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PMID: 8264638 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Binding of TFIID and MEF2 to the TATA element activates transcription of the Xenopus MyoDa promoter.

Molecular and cellular biology ·Vol. 14 ·No. 1 ·1994-01-00 ·Pages 686-99

Leibham D, Wong MW, Cheng TC, Schroeder S, Weil PA, Olson EN, Perry M

Abstract

Members of the MyoD family of helix-loop-helix proteins control expression of the muscle phenotype by regulating the activity of subordinate genes. To investigate processes that control the expression of myogenic factors and regulate the establishment and maintenance of the skeletal muscle phenotype, we have analyzed sequences necessary for transcription of the maternally expressed Xenopus MyoD (XMyoD) gene. A 3.5-kb DNA fragment containing the XMyoDa promoter was expressed in a somite-specific manner in injected frog embryos. The XMyoDa promoter was active in oocytes and cultured muscle cells but not in fibroblasts or nonmuscle cell lines. A 58-bp fragment containing the transcription initiation site, a GC-rich region, and overlapping binding sites for the general transcription factor TFIID and the muscle-specific factor MEF2 was sufficient for muscle-specific transcription. Transcription of the minimal XMyoDa promoter in nonmuscle cells was activated by expression of Xenopus MEF2 (XMEF2) and required binding of both MEF2 and TFIID to the TATA motif. These results demonstrate that the XMyoDa TATA motif is a target for a cell-type-specific regulatory factor and suggests that MEF2 stabilizes and amplifies XMyoDa transcription in mesodermal cells committed to the muscle phenotype.

MeSH Terms
Animals Base Sequence Binding Sites/genetics Cell Differentiation DNA/genetics,metabolism DNA-Binding Proteins/metabolism MEF2 Transcription Factors Molecular Sequence Data Muscles/cytology,metabolism Mutagenesis MyoD Protein/genetics Myogenic Regulatory Factors Promoter Regions, Genetic Sequence Homology, Nucleic Acid TATA Box Tissue Distribution Transcription Factor TFIID Transcription Factors/metabolism Transcriptional Activation Xenopus laevis/genetics,metabolism
Chemicals
DNA-Binding Proteins MEF2 Transcription Factors MyoD Protein Myogenic Regulatory Factors Transcription Factor TFIID Transcription Factors DNA
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Leibham D
Department of Biochemistry and Molecular Biology, University of Texas M. D. Anderson Cancer Center, Houston 77030.
Wong M W
Cheng T C
Schroeder S
Weil P A
Olson E N
Perry M
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.1.686-699.1994
Published
1994-01-00
Pages
686-99
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358418
Subset
IM
Grants
NCI NIH HHS · NCI CA16672 · United States
External Links
PubMed source DOI: 10.1128/mcb.14.1.686-699.1994 PMC Full Text View journal details
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