Home LiteratureArticle Details
PMID: 8251636 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Isolation, sequencing and analysis of the expression of Bryonia calmodulin after mechanical perturbation.

Plant molecular biology ·Vol. 23 ·No. 4 ·1993-11-00 ·Pages 839-46

Galaud JP, Lareyre JJ, Boyer N

Abstract

A cDNA clone (Bc329) encoding calmodulin was isolated from a Bryonia cDNA library by screening with cloned Arabidopsis calmodulin cDNA. The cDNA Bc329 was 899 bp full-length clone. The predicted amino acid sequence consists of 149 residues and reveals a high homology with other known plant calmodulins (91 to 99% identity). Genomic southern blot suggests that Bryonia calmodulin is encoded by a single-copy gene. The Bc329 clone was used as a probe to study the expression of calmodulin mRNA after a mechanical stimulus applied on young Bryonia internodes. The steady-state of calmodulin mRNA reached a maximum 30 min after the treatment before it progressively decreased. The role of calcium and calmodulin as second messengers is discussed with regard to environmental changes.

MeSH Terms
Amino Acid Sequence Base Sequence Calmodulin/genetics Cloning, Molecular Gene Expression Regulation Genes, Plant Molecular Sequence Data Plants/genetics RNA, Messenger/genetics Restriction Mapping Sequence Alignment Sequence Homology, Amino Acid Stress, Mechanical Vegetables/genetics
Chemicals
Calmodulin RNA, Messenger
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Galaud J P
U.A. Physiologie Intégree de l'Arbre Fruitier (INRA-Université), Clermont-Ferrand, France.
Lareyre J J
Boyer N
References (18)
18 references, click to expand
  1. Structure and expression of the Arabidopsis CaM-3 calmodulin gene.
    Plant Mol Biol. 1992 Jul;19(4):649-64 PMID: 1627778
  2. Nucleotide sequence of an alfalfa calmodulin cDNA.
    Nucleic Acids Res. 1990 Jun 11;18(11):3395 PMID: 2356128
  3. Point mutations define a sequence flanking the AUG initiator codon that modulates translation by eukaryotic ribosomes.
    Cell. 1986 Jan 31;44(2):283-92 PMID: 3943125
  4. Rain-, wind-, and touch-induced expression of calmodulin and calmodulin-related genes in Arabidopsis.
    Cell. 1990 Feb 9;60(3):357-64 PMID: 2302732
  5. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  6. Calmodulin genes in trypanosomes are tandemly repeated and produce multiple mRNAs with a common 5' leader sequence.
    Proc Natl Acad Sci U S A. 1985 Jun;82(12):3998-4002 PMID: 3858856
  7. Multiple calmodulin mRNA species are derived from two distinct genes.
    Mol Cell Biol. 1987 May;7(5):1873-80 PMID: 3037336
  8. Turgor-responsive gene transcription and RNA levels increase rapidly when pea shoots are wilted. Sequence and expression of three inducible genes.
    Plant Mol Biol. 1990 Jul;15(1):11-26 PMID: 1715781
  9. Calcium messenger system in plants.
    CRC Crit Rev Plant Sci. 1987;6(1):47-103 PMID: 11540070
  10. Cytological indication of the involvement of calcium and calcium-related proteins in the early responses of Bryonia dioica to mechanical stimulus.
    Protoplasma. 1993;176(3-4):133-7 PMID: 11541041
  11. Multiple divergent mRNAs code for a single human calmodulin.
    J Biol Chem. 1988 Nov 15;263(32):17055-62 PMID: 3182832
  12. Cloning of cDNA Sequences Encoding the Calcium-Binding Protein, Calmodulin, from Barley (Hordeum vulgare L.).
    Plant Physiol. 1989 Jun;90(2):714-9 PMID: 16666833
  13. Molecular cloning and sequencing of a cDNA for plant calmodulin: signal-induced changes in the expression of calmodulin.
    Proc Natl Acad Sci U S A. 1989 May;86(10):3644-8 PMID: 2726741
  14. Regulated expression of the calmodulin-related TCH genes in cultured Arabidopsis cells: induction by calcium and heat shock.
    Proc Natl Acad Sci U S A. 1992 Apr 15;89(8):3213-6 PMID: 1373491
  15. Calmodulin.
    Adv Protein Chem. 1982;35:213-321 PMID: 6762067
  16. Thigmomorphogenesis: the effect of mechanical perturbation on plants.
    Plant Growth Regul. 1993 Feb;12(3):313-24 PMID: 11541741
  17. Primary structures of Arabidopsis calmodulin isoforms deduced from the sequences of cDNA clones.
    Plant Physiol. 1991 Aug;96(4):1196-202 PMID: 16668320
  18. Role of calcium ions in phytochrome responses: an update.
    Physiol Plant. 1986;66:344-8 PMID: 11538657
Article Info
Journal
Plant molecular biology
Abbr.
Plant Mol Biol
ISSN
0167-4412
DOI
10.1007/BF00021538
Published
1993-11-00
Pages
839-46
Language
English
Region
Netherlands
NLM ID
9106343
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00021538 View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2