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PMID: 3037336 Published · ppublish English Comparative Study Journal Article

Multiple calmodulin mRNA species are derived from two distinct genes.

Molecular and cellular biology ·Vol. 7 ·No. 5 ·1987-05-00 ·Pages 1873-80

Nojima H, Kishi K, Sokabe H

Abstract

We have observed three calmodulin mRNA species in rat tissues. In order to know from how many expressed genes they are derived, we have investigated the genomic organization of calmodulin genes in the rat genome. From a rat brain cDNA library, we obtained two kinds of cDNAs (pRCM1 and pRCM3) encoding authentic calmodulin. DNA sequence analysis of these cDNA clones revealed substitutions of nucleotides at 73 positions of 450 nucleotides in the coding region, although the amino acid sequences of these calmodulins are exactly the same. DNA sequences in the 5' and 3' noncoding regions are quite different between these two cDNAs. From these results, we conclude that they are derived from two distinct bona fide calmodulin genes, CaMI (pRCM1) and CaMII (pRCM3). Total genomic Southern hybridization suggested four distinct calmodulin-related genes in the rat genome. By cloning and sequencing the calmodulin-related genes from rat genomic libraries, we demonstrated that the other two genes are processed pseudogenes generated from the CaMI (lambda SC9) and CaMII (lambda SC8) genes, respectively, through an mRNA-mediated process of insertions. Northern blotting showed that the CaMI gene is transcribed in liver, muscle, and brain in similar amounts, whereas the CaMII gene is transcribed mainly in brain. S1 nuclease mapping indicated that the CaMI gene produced two mRNA species (1.7 and 4 kilobases), whereas the CaMII gene expressed a single mRNA species (1.4 kilobases).

MeSH Terms
Animals Base Sequence Brain/physiology Calmodulin/genetics Chromosome Mapping DNA/genetics DNA Restriction Enzymes Endonucleases Gene Expression Regulation Genes Liver/physiology Muscles/physiology RNA, Messenger/genetics Rats Sequence Homology, Nucleic Acid Single-Strand Specific DNA and RNA Endonucleases Tissue Distribution
Chemicals
Calmodulin RNA, Messenger DNA Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Nojima H
Kishi K
Sokabe H
References (30)
30 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.7.5.1873-1880.1987
Published
1987-05-00
Pages
1873-80
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC365291
Subset
IM
Databases
GENBANK
M17068, M17069
External Links
PubMed source DOI: 10.1128/mcb.7.5.1873-1880.1987 PMC Full Text View journal details
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