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PMID: 8248236 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Evidence for myosin motors on organelles in squid axoplasm.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 90 ·No. 23 ·1993-12-01 ·Pages 11252-6

Bearer EL, DeGiorgis JA, Bodner RA, Kao AW, Reese TS

Abstract

Squid axoplasm has proved a rich source for the identification of motors involved in organelle transport. Recently, squid axoplasmic organelles have been shown to move on invisible tracks that are sensitive to cytochalasin, suggesting that these tracks are actin filaments. Here, an assay is described that permits observation of organelles moving on unipolar actin bundles. This assay is used to demonstrate that axoplasmic organelles move on actin filaments in the barbed-end direction, suggesting the presence of a myosin motor on axoplasmic organelles. Indeed, axoplasm contains actin-dependent ATPase activity, and a pan-myosin antibody recognized at least four bands in Western blots of axoplasm. An approximately 235-kDa band copurified in sucrose gradients with KI-extracted axoplasmic organelles, and the myosin antibody stained the organelle surfaces by immunogold electron microscopy. The myosin is present on the surface of at least some axoplasmic organelles and thus may be involved in their transport through the axoplasm, their movement through the cortical actin in the synapse, or some other aspect of axonal function.

MeSH Terms
Acrosome/ultrastructure Actin Cytoskeleton/physiology Actins/physiology Animals Axons/physiology Biological Transport Cell Movement Cytoplasm/metabolism Decapodiformes Enzyme Activation Male Molecular Weight Myosins/chemistry,physiology Organelles/physiology
Chemicals
Actins Myosins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Bearer E L
Division of Biology and Medicine, Brown University, Providence, RI 02912.
DeGiorgis J A
Bodner R A
Kao A W
Reese T S
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.90.23.11252
Published
1993-12-01
Pages
11252-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC47960
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.90.23.11252 PMC Full Text View journal details
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