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PMID: 8226646 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Genes needed for the modification, polymerization, export, and processing of succinoglycan by Rhizobium meliloti: a model for succinoglycan biosynthesis.

Journal of bacteriology ·Vol. 175 ·No. 21 ·1993-11-00 ·Pages 7045-55

Glucksmann MA, Reuber TL, Walker GC

Abstract

The major acidic exopolysaccharide of Rhizobium meliloti, termed succinoglycan, is required for nodule invasion and possibly nodule development. Succinoglycan is a polymer of octasaccharide subunits composed of one galactose residue, seven glucose residues, and acetyl, succinyl, and pyruvyl modifications, which is synthesized on an isoprenoid lipid carrier. A cluster of exo genes in R. meliloti are required for succinoglycan production, and the biosynthetic roles of their gene products have recently been determined (T.L. Reuber and G. C. Walker, Cell 74:269-280, 1993). Our sequencing of 16 kb of this cluster of exo genes and further genetic analysis of this region resulted in the discovery of several new exo genes and has allowed a correlation of the genetic map with the DNA sequence. In this paper we present the sequences of genes that are required for the addition of the succinyl and pyruvyl modifications to the lipid-linked intermediate and genes required for the polymerization of the octasaccharide subunits or the export of succinoglycan. In addition, on the basis of homologies to known proteins, we suggest that ExoN is a uridine diphosphoglucose pyrophosphorylase and that ExoK is a beta(1,3)-beta (1,4)-glucanase. We propose a model for succinoglycan biosynthesis and processing which assigns roles to the products of nineteen exo genes.

Related Genes
exo exoH exoI exoK exoT exoU exoV exoW
MeSH Terms
Amino Acid Sequence Base Sequence Carbohydrate Sequence Chromosome Mapping Conserved Sequence Genes, Bacterial Glycoside Hydrolases/genetics Molecular Sequence Data Multigene Family Mutagenesis, Insertional Open Reading Frames Plasmids Polysaccharides, Bacterial/biosynthesis Sequence Homology, Amino Acid Sinorhizobium meliloti/enzymology,genetics,metabolism UTP-Glucose-1-Phosphate Uridylyltransferase/genetics
Chemicals
Polysaccharides, Bacterial succinoglycan UTP-Glucose-1-Phosphate Uridylyltransferase Glycoside Hydrolases licheninase
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Glucksmann M A
Department of Biology, Massachusetts Institute of Technology, Cambridge 02139.
Reuber T L
Walker G C
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41 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.175.21.7045-7055.1993
Published
1993-11-00
Pages
7045-55
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC206832
Subset
IM
Grants
NIGMS NIH HHS · GM31031 · United States
Databases
GENBANK
L20758
External Links
PubMed source DOI: 10.1128/jb.175.21.7045-7055.1993 PMC Full Text View journal details
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