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PMID: 8209420 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, P.H.S.

Small RNA expression from the oncogenic region of a highly oncogenic strain of herpesvirus saimiri.

Virus genes ·Vol. 8 ·No. 1 ·1994-01-00 ·Pages 25-34

Geck P, Whitaker SA, Medveczky MM, Last TJ, Medveczky PG

Abstract

Herpesvirus saimiri induces acute lymphomas and leukemias in primates and rabbits. Sequence divergence of the right end unique region of the genome classifies virus strains into three groups (A, B, and C), and previous studies have demonstrated correlation between DNA grouping and oncogenicity. In order to relate different oncogenicity to the underlying molecular mechanisms, we reported earlier the expression of a bicistronic mRNA from the oncogenic region in a highly oncogenic group C strain, and the present study is the first report on small RNA transcripts from the same region. The transcripts and 6.2 kbp on the oncogenic region were sequenced and characterized. We show that four U-type small RNAs are expressed in tumor cells transformed by this strain, in contrast to the seven small RNAs reported from a weakly oncogenic group A strain. Sequence comparisons between the two strains showed that the right end region of strain 484-77 of group C is about 1 kbp shorter. The conserved 5' AUUUA repeats of some small RNAs, and their proposed implication in lymphokine mRNA stabilization, are also discussed.

MeSH Terms
Animals Base Sequence Cell Line Cell Transformation, Viral Chromosome Mapping DNA Primers/genetics DNA, Viral/genetics Gene Expression Genes, Viral Herpesviridae Infections/etiology Herpesvirus 2, Saimiriine/genetics,pathogenicity Humans Molecular Sequence Data RNA, Small Nuclear/genetics RNA, Viral/genetics Rabbits Sequence Homology, Nucleic Acid Species Specificity Tumor Virus Infections/etiology
Chemicals
DNA Primers DNA, Viral RNA, Small Nuclear RNA, Viral
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Geck P
Department of Medical Microbiology and Immunology, College of Medicine, University of South Florida, Tampa 33612.
Whitaker S A
Medveczky M M
Last T J
Medveczky P G
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Article Info
Journal
Virus genes
Abbr.
Virus Genes
ISSN
0920-8569
DOI
10.1007/BF01703599
Published
1994-01-00
Pages
25-34
Language
English
Region
United States
NLM ID
8803967
Subset
IM
Grants
PHS HHS · N01 A172644 · United States
NCI NIH HHS · R01 CA53264 · United States
PHSPO CDC HHS · SO7R-R05749 · United States
Databases
GENBANK
X58774
External Links
PubMed source DOI: 10.1007/BF01703599 View journal details
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