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PMID: 820689 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Membranes of Rhodospirillum rubrum: isolation and physicochemical properties of membranes from aerobically grown cells.

Journal of bacteriology ·Vol. 126 ·No. 3 ·1976-06-00 ·Pages 1316-25

Collins ML, Niederman RA

Abstract

Highly purified preparations of cytoplasmic and outer membrane were isolated from aerobically grown Rhodospirillum rubrum lysed by sequential treatment with lysozyme, ethylenediaminetetraacetate, and Brij 58. The membranes were resolved and separated from other cellular constitutents by a combination of velocity and isopyknic sedimentation in sucrose density gradients. On the basis of their appearance in electron micrographs and their protein profiles in sodium dodecyl sulfate-polyacrylamide gel electrophoresis, these preparations appear to be quite similar to those obtained from other gram-negative bacteria. The cytoplasmic membrane fraction contained the majority of the total membrane-bound succinic dehydrogenase activity and was 10-fold enriched in b- and c-type cytochrome with respect to the outer membrane. The latter fraction was characterized by a much greater carbohydrate content and the presence of arachidic acid, which is typical of R. rubrum lipopolysaccharide. Their protein fatty acid, and overall chemical compositions suggested that these preparations were freer from cross-contamination than those obtained from R. rubrum with currently available methods.

MeSH Terms
Aerobiosis Bacterial Proteins/analysis Bacteriochlorophylls/analysis Cell Fractionation Cell Membrane/analysis Cell Wall/analysis Cytochromes/analysis Edetic Acid/pharmacology Fatty Acids/analysis Molecular Weight Muramidase/pharmacology Peptides/analysis Phospholipids/analysis Rhodospirillum rubrum/analysis,growth & development,ultrastructure Surface-Active Agents/pharmacology
Chemicals
Bacterial Proteins Bacteriochlorophylls Cytochromes Fatty Acids Peptides Phospholipids Surface-Active Agents Edetic Acid Muramidase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Collins M L
Niederman R A
References (48)
48 references, click to expand
  1. Identification of an outer membrane protein of Escherichia coli, with a role in the coordination of deoxyribonucleic acid replication and cell elongation.
    J Bacteriol. 1975 Nov;124(2):918-29 PMID: 171254
  2. Membranes of Rhodospirillum rubrum: physicochemical properties of chromatophore fractions isolated from osmotically and mechanically disrupted cells.
    J Bacteriol. 1976 Jun;126(3):1326-38 PMID: 820690
  3. Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli.
    J Bacteriol. 1970 Nov;104(2):890-901 PMID: 4099097
  4. Isolation and fractionation of the photosynthetic membranous organelles from Rhodopseudomonas spheroides.
    J Bacteriol. 1971 Oct;108(1):465-73 PMID: 4107812
  5. [The bacteriochlorophyll content and protein composition of thylakoids of Rhodospirillum rubrum during morphogenesis of the photosynthetic apparatus].
    Arch Mikrobiol. 1969;66(2):154-65 PMID: 4194589
  6. Separation and properties of outer and cytoplasmic membranes in Escherichia coli.
    Biochim Biophys Acta. 1969;193(2):268-76 PMID: 4242764
  7. Bacterial cytochromes.
    Annu Rev Microbiol. 1968;22:181-200 PMID: 4301025
  8. Topology and growth of the intracytoplasmic membrane system of Rhodopseudomonas spheroides: protein, chlorophyll, and phospholipid insertion into steady-state anaerobic cells.
    J Bacteriol. 1974 Jun;118(3):1144-57 PMID: 4545399
  9. Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.
    J Biol Chem. 1972 Jun 25;247(12):3962-72 PMID: 4555955
  10. Proteins of the inner membrane of Escherichia coli: identification of succinate dehydrogenase by polyacrylamide gel electrophoresis with sdh amber mutants.
    J Bacteriol. 1974 Mar;117(3):947-53 PMID: 4591960
  11. Separation by density gradient centrifugation of two types of membranes from spheroplast membrane of Escherichia coli K12.
    Biochim Biophys Acta. 1968 Jan 3;150(1):159-61 PMID: 4868245
  12. Bacterial cytochromes. I. Structural aspects.
    Annu Rev Biochem. 1970;39:673-700 PMID: 4920828
  13. Production and ultrastructure of lysozyme and ethylenediaminetetraacetate-lysozyme spheroplasts of Escherichia coli.
    J Bacteriol. 1967 Jan;93(1):427-37 PMID: 4960155
  14. The separation of chromatophores from the cell envelope in Rhodopseudomonas spheroides.
    Prep Biochem. 1971;1(2):141-50 PMID: 5317366
  15. Isolation and characterization of the membranes from Rhodospirillum rubrum.
    Biochim Biophys Acta. 1970;196(2):141-61 PMID: 5414301
  16. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  17. Light-dependent utilization of organic compounds and photoproduction of molecular hydrogen by photosynthetic bacteria; relationships with nitrogen metabolism.
    Arch Biochem Biophys. 1961 Sep;94:449-63 PMID: 13731247
  18. The fine structure of Rhodospirillum rubrum.
    J Cell Biol. 1963 Feb;16:401-19 PMID: 14022119
  19. TOWARD THE ISOLATION OF A PHOTOCHEMICAL REACTION CENTER IN RHODOPSEUDOMONAS SPHEROIDES.
    Biochim Biophys Acta. 1963 Nov 29;75:312-23 PMID: 14104940
  20. Comparison of the envelope protein compositions of several gram-negative bacteria.
    J Bacteriol. 1970 Dec;104(3):1404-5 PMID: 16559124
  21. Localization of membrane proteins in membrane vesicles of Bacillus subtilis.
    Arch Biochem Biophys. 1975 Apr;167(2):570-80 PMID: 235900
  22. Characterization of two cell-envelope fractions from chemotrophically grown Rhodospirillum rubrum.
    Antonie Van Leeuwenhoek. 1975;41(3):273-86 PMID: 813575
  23. The outer membrane of Proteus mirabilis. I. Isolation and characterization of the outer and cytoplasmic membrane fractions.
    Biochim Biophys Acta. 1975 Feb 14;375(3):381-94 PMID: 1091289
  24. Dissociation and reassembly of Escherichia coli outer membrane and of lipopolysaccharide, and their reassembly onto flagellar basal bodies.
    J Bacteriol. 1971 Mar;105(3):1184-99 PMID: 4100836
  25. Membrane differentiation in phototrophically growing Rhodospirillum rubrum during transition from low to high light intensity.
    Biochim Biophys Acta. 1973 Nov 30;330(1):80-9 PMID: 4148662
  26. Release of lipopolysaccharide by EDTA treatment of E. coli.
    Biochem Biophys Res Commun. 1965 Nov 22;21(4):290-6 PMID: 4159978
  27. Genetic and biochemical characterization of mutants of Bacillus subtilis defective in succinate dehydrogenase.
    J Bacteriol. 1973 Sep;115(3):738-45 PMID: 4199512
  28. Attachment of flagellar basal bodies to the cell envelope: specific attachment to the outer, lipopolysaccharide membrane and the cyoplasmic membrane.
    J Bacteriol. 1971 Jan;105(1):396-407 PMID: 4250610
  29. Observations on distribution of NADH oxidase in particles from dark-grown and light-grown Rhodospirillum rubrum.
    Biochem Biophys Res Commun. 1969 Feb 21;34(4):418-25 PMID: 4304836
  30. Isolation and properties of succinate dehydrogenase from Rhodospirillum rubrum.
    Arch Biochem Biophys. 1972 Oct;152(2):613-8 PMID: 4344128
  31. Membranes of Rhodopseudomonas spheroides. I. Isolation and characterization of membrane fractions from extracts of aerobically and anaerobically grown cells.
    Arch Biochem Biophys. 1972 Oct;152(2):547-60 PMID: 4539052
  32. Distribution of lipids in the wall and cytoplasmic membrane subfractions of the cell envelope of Escherichia coli.
    J Bacteriol. 1972 Feb;109(2):686-90 PMID: 4550815
  33. Outer membrane proteins of Escherichia coli. I. Effect of preparative conditions on the migration of protein in polyacrylamide gels.
    Arch Biochem Biophys. 1973 Aug;157(2):541-52 PMID: 4581236
  34. Purification of cytoplasmic membranes and outer membranes from Proteus mirabilis.
    Arch Mikrobiol. 1973 Nov 19;93(4):311-25 PMID: 4591534
  35. Membranes of Rhodopseudomonas spheroides: interactions of chromatophores with the cell envelope.
    J Bacteriol. 1974 Jan;117(1):19-28 PMID: 4808899
  36. A low-viscosity epoxy resin embedding medium for electron microscopy.
    J Ultrastruct Res. 1969 Jan;26(1):31-43 PMID: 4887011
  37. Examination of the protein composition of the cell envelope of Escherichia coli by polyacrylamide gel electrophoresis.
    J Bacteriol. 1970 Nov;104(2):882-9 PMID: 4923077
  38. Lysis of Escherichia coli with a neutral detergent.
    Biochim Biophys Acta. 1967 Dec 19;149(2):476-88 PMID: 4966087
  39. Effect of ethylenediaminetetraacetic acid, Triton X-100, and lysozyme on the morphology and chemical composition of isolate cell walls of Escherichia coli.
    J Bacteriol. 1971 Oct;108(1):553-63 PMID: 5001205
  40. [Fatty acid composition of whole cells, thylakoids and lipopolysaccharides of Rhodospirillum rubrum and Rhodopseudomonas capsulata].
    Arch Mikrobiol. 1969;66(3):273-80 PMID: 5384698
  41. A model for the behavior of vesicles in density gradients: implications for fractionation.
    Biochim Biophys Acta. 1970 Jun 2;203(3):385-93 PMID: 5523738
  42. Studies on the macro-molecular organization of microbial cells.
    Arch Biochem Biophys. 1952 Jul;38:245-60 PMID: 12997099
  43. Kinetic studies of pigment synthesis by non-sulfur purple bacteria.
    J Cell Physiol. 1957 Feb;49(1):25-68 PMID: 13416343
  44. Observations on the relationship between the formation of photopigments and the synthesis of protein in Rhodopseudomonas spheroides.
    J Gen Microbiol. 1962 Sep;28:599-605 PMID: 13913484
  45. RECONSTITUTION OF RESPIRATORY CHAIN ENZYME SYSTEMS. XI. USE OF ARTIFICIAL ELECTRON ACCEPTORS IN THE ASSAY OF SUCCINATE-DEHYDROGENATING ENZYMES.
    J Biol Chem. 1963 Dec;238:4032-6 PMID: 14086742
  46. STUDIES OF ESCHERICHIA COLI RIBONUCLEIC ACID-DEOXYRIBONUCLEIC ACID COMPLEX.
    Biochim Biophys Acta. 1965 May 11;103:60-9 PMID: 14336443
  47. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  48. SOME OBSERVATIONS ON THE SYNTHESIS AND FUNCTION OF THE PHOTOSYNTHETIC APPARATUS IN RHODOSPIRILLUM RUBRUM.
    Proc Natl Acad Sci U S A. 1960 Dec;46(12):1543-53 PMID: 16590780
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.126.3.1316-1325.1976
Published
1976-06-00
Pages
1316-25
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC233158
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.126.3.1316-1325.1976 PMC Full Text View journal details
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