Distribution of lipids in the wall and cytoplasmic membrane subfractions of the cell envelope of Escherichia coli.

Journal of bacteriology ·Vol. 109 ·No. 2 ·1972-02-00 ·Pages 686-90

White DA, Lennarz WJ, Schnaitman CA

Abstract

Cell wall and membrane subfractions of the cell envelope of Escherichia coli have been isolated by a procedure involving particle electrophoresis and sucrose gradient density centrifugation. The lipid content of each fraction has been investigated. The individual phospholipids of both fractions are quantitatively similar except that the proportion of lysophosphatidylethanolamine is greater in the wall than in the membrane. Fatty acid analysis of the phospholipids of each fraction revealed that the wall phospholipids contain a greater proportion of palmitic acid. Coenzyme Q is almost exclusively localized in the cell membrane.

MeSH Terms

Carbon Isotopes Cell Fractionation Cell Membrane/analysis Cell Wall/analysis Centrifugation, Density Gradient Chromatography, Gas Chromatography, Thin Layer Coenzymes Electrophoresis Escherichia coli/analysis,cytology,enzymology,growth & development Fatty Acids/analysis Galactose/metabolism Leucine/metabolism Lipids/analysis Lipopolysaccharides/analysis Palmitic Acids/analysis Phosphatidylethanolamines/analysis Phospholipids/analysis Spectrophotometry Succinate Dehydrogenase/metabolism Tritium Tyrosine/metabolism Ubiquinone/analysis

Chemicals

Carbon Isotopes Coenzymes Fatty Acids Lipids Lipopolysaccharides Palmitic Acids Phosphatidylethanolamines Phospholipids Tritium Ubiquinone Tyrosine Succinate Dehydrogenase Leucine Galactose

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

White D A

Lennarz W J

Schnaitman C A

References (19)

19 references, click to expand

Protein measurement with the Folin phenol reagent.
J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
The structure of the naturally occurring phosphoglycerides. III. Action of moccasin-venom phospholipase A on ovolecithin and related substances.
Biochem J. 1957 Feb;65(2):382-9 PMID: 13403920
Studies on the electron transport system. XX. Chemical and physical properties of the coenzyme Q family of compounds.
Biochim Biophys Acta. 1959 May;33(1):169-85 PMID: 13651197
The natural occurrence of coenzyme Q and related compounds.
J Biol Chem. 1959 Aug;234(8):2169-75 PMID: 13673033
The rapid incorporation of phosphate into mitochondrial lipids.
J Biol Chem. 1963 Jan;238:59-63 PMID: 13946475
PURIFICATION AND CHARACTERIZATION OF THE LIPID A COMPONENT OF THE LIPOPOLYSACCHARIDES FROM ESCHERICHIA COLI.
Biochemistry. 1964 Mar;3:411-8 PMID: 14155106
COMPARISON OF LIPIDS AND LIPOPOLYSACCHARIDE FROM THE BACILLARY AND L FORMS OF PROTEUS P18.
J Bacteriol. 1965 Apr;89:1020-5 PMID: 14276089
Limitations of the phenazine methosulphate assay for succinic and related dehydrogenases.
Nature. 1962 Mar 31;193:1256-8 PMID: 13862582
THE BIOSYNTHESIS OF CELL WALL LIPOPOLYSACCHARIDE IN ESCHERICHIA COLI. I. THE BIOCHEMICAL PROPERTIES OF A URIDINE DIPHOSPHATE GALACTOSE 4-EPIMERASELESS MUTANT.
J Biol Chem. 1965 May;240:1919-25 PMID: 14299610
The role of shikimic acid in the biosynthesis of vitamin K2.
Biochem J. 1966 Jul;100(1):1-6 PMID: 5337721
Separation by density gradient centrifugation of two types of membranes from spheroplast membrane of Escherichia coli K12.
Biochim Biophys Acta. 1968 Jan 3;150(1):159-61 PMID: 4868245
An equilibrium between two fractions of lipopolysaccharide in Escherichia coli.
Proc Natl Acad Sci U S A. 1968 Dec;61(4):1435-9 PMID: 4974319
Structure and biosynthesis of the bacterial cell wall.
Annu Rev Biochem. 1969;38:501-38 PMID: 4896244
Structure of Escherichia coli after freeze-etching.
J Bacteriol. 1970 Jan;101(1):304-13 PMID: 4189229
The surface charge of rat liver mitochondria and their membranes. Clarification of some controversies concerning mitochondrial structure.
J Cell Biol. 1970 Jul;46(1):137-50 PMID: 4318844
Examination of the protein composition of the cell envelope of Escherichia coli by polyacrylamide gel electrophoresis.
J Bacteriol. 1970 Nov;104(2):882-9 PMID: 4923077
Protein composition of the cell wall and cytoplasmic membrane of Escherichia coli.
J Bacteriol. 1970 Nov;104(2):890-901 PMID: 4099097
Zymogen secretion and phospholipid metabolism in the pancreas. Phospholipids of the zymogen granule.
Biochem J. 1970 Dec;120(3):533-8 PMID: 5499965
Solubilization of the cytoplasmic membrane of Escherichia coli by Triton X-100.
J Bacteriol. 1971 Oct;108(1):545-52 PMID: 4941569

Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.109.2.686-690.1972

Published

1972-02-00

Pages

686-90

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC285193

Subset

External Links

PubMed source DOI: 10.1128/jb.109.2.686-690.1972 PMC Full Text View journal details

Analysis Services

Distribution of lipids in the wall and cytoplasmic membrane subfractions of the cell envelope of Escherichia coli.

Contact

WeChat Official Account