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PMID: 5499965 Published · ppublish English Journal Article

Zymogen secretion and phospholipid metabolism in the pancreas. Phospholipids of the zymogen granule.

The Biochemical journal ·Vol. 120 ·No. 3 ·1970-12-00 ·Pages 533-8

White DA, Hawthorne JN

Abstract

1. Acetylcholine produced a fivefold increase in the specific radioactivity of phosphatidylinositol in guinea-pig pancreas slices incubated in the presence of [(32)P]-orthophosphate, but did not affect the specific radioactivity of the terminal phosphate of ATP. 2. The secretagogues acetylcholine and pancreozymin had no direct effect on the enzymes of phosphatidylinositol biosynthesis in guinea-pig pancreas homogenates. 3. Pancreozymin in vivo increased the incorporation of [(32)P]orthophosphate into phosphatidylinositol but not into phosphatidylcholine, phosphatidylethanolamine or phosphatidylserine. 4. The zymogen-granule membrane contained no unusual phospholipids or lysophospholipids, although its high cholesterol/phospholipid ratio resembled that of plasma membrane. 5. Phospholipase A activity during fractionation sometimes produced lysophospholipids in mitochondrial, microsomal and zymogen-granule fractions.

MeSH Terms
Acetylcholine/pharmacology Adenosine Triphosphate/metabolism Animals Cattle Cell Membrane Cholesterol/metabolism Cytoplasmic Granules/enzymology Phosphatidylethanolamines/metabolism
Chemicals
Phosphatidylethanolamines Adenosine Triphosphate Cholesterol Acetylcholine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
White D A
Hawthorne J N
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34 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1200533
Published
1970-12-00
Pages
533-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1179634
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1200533 PMC Full Text View journal details
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