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PMID: 4293959 Published · ppublish English Journal Article

The biosynthesis of phosphatidic acid and phosphatidylinositol in mammalian pancreas.

The Biochemical journal ·Vol. 105 ·No. 1 ·1967-10-00 ·Pages 379-92

Prottey C, Hawthorne JN

Abstract

1. The labelling of guinea-pig pancreas phospholipids in vivo after intraperitoneal injection of [(32)P]orthophosphate is described. 2. Acyl-CoA synthetase activity in pancreas homogenates has been studied. There is no absolute requirement for added fatty acids, indicating an adequate supply of endogenous fatty acids in these preparations. 3. Phosphatidic acid is formed in guinea-pig pancreas preparations by two distinct routes, namely the acylation of l-3-glycerophosphate and the phosphorylation of 1,2-diglyceride. Phosphatidic acid formed by either mechanism is converted into phosphatidylinositol by guinea-pig pancreas in vitro. 4. The enzymes of pancreas that convert phosphatidic acid into phosphatidylinositol via CDP-diglyceride have been characterized. 5. Addition of bovine serum albumin is necessary in assaying certain of these enzymes.

MeSH Terms
Animals Chromatography, Paper Fatty Acids/metabolism Glycerophosphates/metabolism Guinea Pigs Ligases/metabolism Models, Biological Nucleotides Pancreas/enzymology,metabolism Phosphates/metabolism Phosphatidylinositols/biosynthesis Phospholipids/biosynthesis Phosphorus Isotopes Proteins/metabolism Serum Albumin, Bovine
Chemicals
Fatty Acids Glycerophosphates Nucleotides Phosphates Phosphatidylinositols Phospholipids Phosphorus Isotopes Proteins Serum Albumin, Bovine Ligases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Prottey C
Hawthorne J N
References (36)
36 references, click to expand
  1. Enzymatic synthesis of the coenzyme A derivatives of long chain fatty acids.
    J Biol Chem. 1953 Sep;204(1):329-43 PMID: 13084605
  2. THE ENZYMATIC ACYLATION OF LYSOPHOSPHATIDYLINOSITOL.
    J Biol Chem. 1964 Jul;239:2123-9 PMID: 14209937
  3. Respiration and phosphorylation in preparations from Mammalian Brain.
    Biochem J. 1951 Jan;48(1):1-11 PMID: 14820776
  4. Biosynthesis of lipids in plants. II. Incorporation of glycerophosphate-32-P into phosphatides by cell-free preparations from spinach leaves.
    Can J Biochem. 1966 Apr;44(4):459-67 PMID: 5959905
  5. The enzymatic synthesis of inositol phosphatide.
    J Biol Chem. 1958 Nov;233(5):1077-83 PMID: 13598735
  6. SPECIFIC ASSAYS OF SOME PHOSPHATASES IN SUBCELLULAR FRACTIONS OF SMALL INTESTINAL MUCOSA.
    Nature. 1965 Feb 20;205:799-800 PMID: 14292314
  7. On the conversion of fructose to glucose by guinea pig intestine.
    Biochim Biophys Acta. 1960 Mar 11;38:427-34 PMID: 13850248
  8. Metabolism of phospholipids. 8. Biosynthesis of phosphatidylcholine in the intestinal mucosa.
    Biochim Biophys Acta. 1965 Jun 1;98(3):486-501 PMID: 4284412
  9. Pancreatic microsomes; an integrated morphological and biochemical study.
    J Biophys Biochem Cytol. 1956 Nov 25;2(6):671-90 PMID: 13398437
  10. The colorimetric determination of phosphorus.
    Biochem J. 1932;26(2):292-7 PMID: 16744823
  11. IONIC PROPERTIES OF AQUEOUS DISPERSIONS OF PHOSPHATIDIC ACID.
    J Biol Chem. 1964 Dec;239:4066-72 PMID: 14247649
  12. The synthesis of phosphatidic acid from diglyceride and adenosine triphosphate in extracts of brain microsomes.
    J Biol Chem. 1959 Jun;234(6):1381-6 PMID: 13654382
  13. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat-liver tissue.
    Biochem J. 1955 Aug;60(4):604-17 PMID: 13249955
  14. A simple method for the preparation of 32-P-labelled adenosine triphosphate of high specific activity.
    Biochem J. 1964 Jan;90(1):147-9 PMID: 5832284
  15. The effect of palmityl coenzyme A on glucose 6-phosphate dehydrogenase and other enzymes.
    J Biol Chem. 1966 Feb 10;241(3):720-6 PMID: 4286183
  16. The lipids of mammalian pancreas.
    Biochem J. 1966 Oct;101(1):191-6 PMID: 5971782
  17. Effects of acetylcholine on the turnover of phosphoryl units in individual phospholipids of pancreas slices and brain cortex slices.
    Biochim Biophys Acta. 1955 Sep;18(1):102-10 PMID: 13260248
  18. Diglyceride kinase and phosphatidic acid phosphatase in erythrocyte membranes.
    Nature. 1961 Mar 11;189:836-7 PMID: 13715208
  19. The enzymatic synthesis of inositol monophosphatide.
    J Biol Chem. 1960 May;235:1303-11 PMID: 14431034
  20. Metabolism of phospholipids. II. Isolation and properties of phosphatidic acid from mammalian liver.
    Biochim Biophys Acta. 1960 Jun 17;41:45-54 PMID: 14403714
  21. Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase.
    Biochem J. 1961 Sep;80:649-54 PMID: 13734134
  22. The structure of the naturally occurring phosphoglycerides. 4. Action of cabbage-leaf phospholipase D on ovolecithin and related substances.
    Biochem J. 1958 Jul;69(3):458-66 PMID: 13560391
  23. Incorporation of injected [32P] phosphate into the phosphoinositides of subcellular fractions from young rat brain.
    Biochem J. 1966 Jan;98(1):62-7 PMID: 4287185
  24. Assay of glycerol phosphate acyltransferase in liver particles.
    Biochem J. 1963 Feb;86:244-7 PMID: 14014948
  25. Preparation of human serum albumin free of long-chain fatty acids.
    Science. 1957 Jun 28;125(3261):1296-7 PMID: 13432802
  26. METABOLISM OF GLYCEROLIPIDS. VI. SPECIFICITIES OF ACYL COENZYME A: PHOSPHOLIPID ACYLTRANSFERASES.
    J Biol Chem. 1965 May;240:1905-11 PMID: 14299609
  27. The phospholipids of liver-cell fractions. II. Incorporation of [32P]orthophosphate in vivo in normal and regenerating rat liver.
    Biochim Biophys Acta. 1965 Oct 4;106(2):357-70 PMID: 5867696
  28. Studies on the role of phospholipids in phagocytosis.
    J Biol Chem. 1966 Jul 25;241(14):3354-61 PMID: 5913125
  29. Metabolism of phospholipids. V. Studies of phosphatidic acid phosphatase.
    Biochim Biophys Acta. 1962 Jan 29;56:479-90 PMID: 13880482
  30. A simple method for the isolation and purification of total lipides from animal tissues.
    J Biol Chem. 1957 May;226(1):497-509 PMID: 13428781
  31. Effects of calcium omission on acetylcholine-stimulated amylase secretion and phospholipid synthesis in pigeon pancreas slices.
    Biochim Biophys Acta. 1966 Jan 25;115(1):219-21 PMID: 4286997
  32. Enzymatic synthesis of cytidine diphosphate diglyceride.
    J Lipid Res. 1966 Sep;7(5):678-83 PMID: 4291255
  33. Biosynthesis of phosphatidylinositol in rat brain.
    Biochem J. 1963 Apr;87:136-42 PMID: 13981209
  34. Studies on the acetylcholine-stimulated incorporation of radioactive inorganic orthophosphate into the phospholipid of brain particulate preparations. II. Subcellular distribution of enzymic activity.
    J Neurochem. 1966 Jun;13(6):487-91 PMID: 4288042
  35. A hydrolytic procedure for the identification and estimation of individual phospholipids in biological samples.
    Biochem J. 1960 Apr;75:45-53 PMID: 13814585
  36. Enzymatic esterification of alpha-glycerophosphate by long chain fatty acids.
    J Biol Chem. 1953 Sep;204(1):345-57 PMID: 13084606
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1050379
Published
1967-10-00
Pages
379-92
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1198310
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1050379 PMC Full Text View journal details
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