Induction of apoptosis in a T lymphoblastoid cell line infected with feline immunodeficiency virus.

Archives of virology ·Vol. 135 ·No. 1-2 ·1994-00-00 ·Pages 153-8

Ohno K, Okamoto Y, Miyazawa T, Mikami T, Watari T, Goitsuka R, Tsujimoto H, Hasegawa A

Abstract

The mechanism of cell death induced by feline immunodeficiency virus (FIV) infection was investigated in an interleukin 2(IL-2)-dependent T-lymphoblastoid cell line (MYA-1). DNA extracted from FIV-infected MYA-1 cells showed a ladder of nucleosomal DNA, indicating that the cytopathic effect (CPE) observed in these cells was due to apoptosis. Infection of MYA-1 cells with FIV was associated with suppression of the proliferative response of the cells to exogenous IL-2 prior to DNA fragmentation. These findings suggest that FIV-induced CPE in these T-lymphoblastoid cells is associated with apoptosis possibly due to a defect in the IL-2 signal transduction pathway.

MeSH Terms

Animals Apoptosis Cats Cell Division/drug effects Cell Line DNA Damage Flow Cytometry Humans Immunodeficiency Virus, Feline/pathogenicity,physiology Interleukin-2/pharmacology Receptors, Interleukin-2/biosynthesis Recombinant Proteins/pharmacology T-Lymphocytes/cytology,microbiology

Chemicals

Interleukin-2 Receptors, Interleukin-2 Recombinant Proteins

Authors & Affiliations

8 authors, click to expand affiliations / ORCID

Ohno K

Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.

Okamoto Y

Miyazawa T

Mikami T

Watari T

Goitsuka R

Tsujimoto H

Hasegawa A

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Article Info

Journal

Archives of virology

Abbr.

Arch Virol

ISSN

0304-8608

DOI

10.1007/BF01309772

Published

1994-00-00

Pages

153-8

Language

English

Region

Austria

NLM ID

7506870

Subset

External Links

PubMed source DOI: 10.1007/BF01309772 View journal details

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Induction of apoptosis in a T lymphoblastoid cell line infected with feline immunodeficiency virus.

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