Home LiteratureArticle Details
PMID: 8196655 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Homologous recombination of monkey alpha-satellite repeats in an in vitro simian virus 40 replication system: possible association of recombination with DNA replication.

Molecular and cellular biology ·Vol. 14 ·No. 6 ·1994-06-00 ·Pages 4173-82

Kawasaki I, Bae YS, Eki T, Kim Y, Ikeda H

Abstract

To study homologous recombination between repeated sequences in an in vitro simian virus 40 (SV40) replication system, we constructed a series of substrate DNAs that contain two identical fragments of monkey alpha-satellite repeats. Together with the SV40-pBR322 composite vector encoding Apr and Kmr, the DNAs also contain the Escherichia coli galactokinase gene (galK) positioned between two alpha-satellite fragments. The alpha-satellite sequence used consists of multiple units of tandem 172-bp sequences which differ by microheterogeneity. The substrate DNAs were incubated in an in vitro SV40 DNA replication system and used to transform the E. coli galK strain DH10B after digestion with DpnI. The number of E. coli galK Apr Kmr colonies which contain recombinant DNAs were determined, and their structures were analyzed. Products of equal and unequal crossovers between identical 172-bp sequences and between similar but not identical (homeologous) 172-bp sequences, respectively, were detected, although those of the equal crossover were predominant among all of the galK mutant recombinants. Similar products were also observed in the in vivo experiments with COS1 cells. The in vitro experiments showed that these recombinations were dependent on the presence of both the SV40 origin of DNA replication and SV40 large T antigen. Most of the recombinant DNAs were generated from newly synthesized DpnI-resistant DNAs. These results suggest that the homologous recombination observed in this SV40 system is associated with DNA replication and is suppressed by mismatches in heteroduplexes formed between similar but not identical sequences.

Related Genes
galK
MeSH Terms
Animals Antigens, Polyomavirus Transforming/genetics Base Sequence Crossing Over, Genetic DNA Replication DNA, Satellite/genetics,metabolism Escherichia coli/enzymology,genetics Galactokinase/biosynthesis,genetics Genes, Bacterial Haplorhini Kinetics Models, Genetic Molecular Sequence Data Plasmids Recombination, Genetic Repetitive Sequences, Nucleic Acid Restriction Mapping Simian virus 40/genetics
Chemicals
Antigens, Polyomavirus Transforming DNA, Satellite Galactokinase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Kawasaki I
Department of Molecular Biology, University of Tokyo, Japan.
Bae Y S
Eki T
Kim Y
Ikeda H
References (47)
47 references, click to expand
  1. Instability of a 550-base pair DNA segment and abnormal methylation in fragile X syndrome.
    Science. 1991 May 24;252(5009):1097-102 PMID: 2031184
  2. In vitro rescue of an integrated hybrid adeno-associated virus/simian virus 40 genome.
    J Mol Biol. 1991 Apr 20;218(4):791-804 PMID: 1850805
  3. Mapping of DNA instability at the fragile X to a trinucleotide repeat sequence p(CCG)n.
    Science. 1991 Jun 21;252(5013):1711-4 PMID: 1675488
  4. A shuttle vector for analysis of illegitimate recombination in mammalian cells: effects of DNA topoisomerase inhibitors on deletion frequency.
    Gene. 1991 May 30;101(2):285-9 PMID: 1647363
  5. Androgen receptor gene mutations in X-linked spinal and bulbar muscular atrophy.
    Nature. 1991 Jul 4;352(6330):77-9 PMID: 2062380
  6. Molecular mechanisms of deletion formation in Escherichia coli plasmids. I. Deletion formation mediated by long direct repeats.
    Mol Gen Genet. 1991 Aug;228(1-2):153-9 PMID: 1679524
  7. A new type of insertion mutation in monkey cells: insertion accompanied by long target site duplication.
    Mol Gen Genet. 1991 Oct;229(3):325-33 PMID: 1658594
  8. Variation of the CGG repeat at the fragile X site results in genetic instability: resolution of the Sherman paradox.
    Cell. 1991 Dec 20;67(6):1047-58 PMID: 1760838
  9. Molecular basis of myotonic dystrophy: expansion of a trinucleotide (CTG) repeat at the 3' end of a transcript encoding a protein kinase family member.
    Cell. 1992 Feb 21;68(4):799-808 PMID: 1310900
  10. Two alternative pathways of double-strand break repair that are kinetically separable and independently modulated.
    Mol Cell Biol. 1992 Mar;12(3):1292-303 PMID: 1545810
  11. Myotonic dystrophy mutation: an unstable CTG repeat in the 3' untranslated region of the gene.
    Science. 1992 Mar 6;255(5049):1253-5 PMID: 1546325
  12. An unstable triplet repeat in a gene related to myotonic muscular dystrophy.
    Science. 1992 Mar 6;255(5049):1256-8 PMID: 1546326
  13. Cloning and sequencing of Escherichia coli mutR shows its identity to topB, encoding topoisomerase III.
    J Bacteriol. 1992 Aug;174(15):5168-70 PMID: 1321123
  14. Recombination between similar but not identical DNA sequences during yeast transformation occurs within short stretches of identity.
    Cell. 1992 Aug 21;70(4):659-70 PMID: 1505030
  15. Identification of an Alu retrotransposition event in close proximity to a strong candidate gene for Huntington's disease.
    Nature. 1993 Mar 25;362(6418):370-3 PMID: 8384324
  16. Clues to the pathogenesis of familial colorectal cancer.
    Science. 1993 May 7;260(5109):812-6 PMID: 8484121
  17. Ubiquitous somatic mutations in simple repeated sequences reveal a new mechanism for colonic carcinogenesis.
    Nature. 1993 Jun 10;363(6429):558-61 PMID: 8505985
  18. The human mutator gene homolog MSH2 and its association with hereditary nonpolyposis colon cancer.
    Cell. 1993 Dec 3;75(5):1027-38 PMID: 8252616
  19. Hypermutability and mismatch repair deficiency in RER+ tumor cells.
    Cell. 1993 Dec 17;75(6):1227-36 PMID: 8261516
  20. Selective extraction of polyoma DNA from infected mouse cell cultures.
    J Mol Biol. 1967 Jun 14;26(2):365-9 PMID: 4291934
  21. Unequal meiotic recombination within tandem arrays of yeast ribosomal DNA genes.
    Cell. 1980 Mar;19(3):765-74 PMID: 6988084
  22. SV40 DNA transfection of cells in suspension: analysis of efficiency of transcription and translation of T-antigen.
    Gene. 1981 Mar;13(2):197-202 PMID: 6263759
  23. On the formation of spontaneous deletions: the importance of short sequence homologies in the generation of large deletions.
    Cell. 1982 Jun;29(2):319-28 PMID: 6288254
  24. Model for homologous recombination during transfer of DNA into mouse L cells: role for DNA ends in the recombination process.
    Mol Cell Biol. 1984 Jun;4(6):1020-34 PMID: 6330525
  25. Replication and recombination in adenovirus-infected cells are temporally and functionally related.
    J Virol. 1984 Sep;51(3):571-7 PMID: 6471165
  26. Recombination in adenovirus: analysis of crossover sites in intertypic overlap recombinants.
    Virology. 1984 Nov;139(1):43-52 PMID: 6495658
  27. The minimum amount of homology required for homologous recombination in mammalian cells.
    Mol Cell Biol. 1984 Nov;4(11):2253-8 PMID: 6096689
  28. In vitro replication of duplex circular DNA containing the simian virus 40 DNA origin site.
    Proc Natl Acad Sci U S A. 1985 Sep;82(17):5710-4 PMID: 2994044
  29. Homologous recombination in Escherichia coli: dependence on substrate length and homology.
    Genetics. 1986 Mar;112(3):441-57 PMID: 3007275
  30. Heteroduplex deoxyribonucleic acid base mismatch repair in bacteria.
    Microbiol Rev. 1986 Jun;50(2):133-65 PMID: 3523187
  31. Homology requirement for efficient gene conversion between duplicated chromosomal sequences in mammalian cells.
    Genetics. 1987 Jan;115(1):161-7 PMID: 3557108
  32. Differential effects of base-pair mismatch on intrachromosomal versus extrachromosomal recombination in mouse cells.
    Proc Natl Acad Sci U S A. 1987 Aug;84(15):5340-4 PMID: 3037544
  33. Meiotic recombination between repeated transposable elements in Saccharomyces cerevisiae.
    Mol Cell Biol. 1988 Jul;8(7):2942-54 PMID: 2841590
  34. Mitotic recombination in the rDNA of S. cerevisiae is suppressed by the combined action of DNA topoisomerases I and II.
    Cell. 1988 Nov 4;55(3):413-25 PMID: 2902925
  35. A new role for a yeast transcriptional silencer gene, SIR2, in regulation of recombination in ribosomal DNA.
    Cell. 1989 Mar 10;56(5):771-6 PMID: 2647300
  36. A subthreshold level of DNA topoisomerases leads to the excision of yeast rDNA as extrachromosomal rings.
    Cell. 1989 Jun 16;57(6):975-85 PMID: 2544296
  37. A hyper-recombination mutation in S. cerevisiae identifies a novel eukaryotic topoisomerase.
    Cell. 1989 Jul 28;58(2):409-19 PMID: 2546682
  38. Genetic and physical analysis of double-strand break repair and recombination in Saccharomyces cerevisiae.
    Genetics. 1989 Jul;122(3):519-34 PMID: 2668114
  39. Double-strand breaks stimulate alternative mechanisms of recombination repair.
    J Mol Biol. 1989 Jun 5;207(3):527-41 PMID: 2668534
  40. Effect of base pair mismatches on recombination via the RecBCD pathway.
    Mol Gen Genet. 1989 Aug;218(2):358-60 PMID: 2550771
  41. The barrier to recombination between Escherichia coli and Salmonella typhimurium is disrupted in mismatch-repair mutants.
    Nature. 1989 Nov 23;342(6248):396-401 PMID: 2555716
  42. Intermolecular recombination between DNAs introduced into mouse L cells is mediated by a nonconservative pathway that leads to crossover products.
    Mol Cell Biol. 1990 Jan;10(1):103-12 PMID: 2294396
  43. Hypervariable minisatellite DNA is a hotspot for homologous recombination in human cells.
    Cell. 1990 Jan 12;60(1):95-103 PMID: 2295091
  44. The Z-DNA motif d(TG)30 promotes reception of information during gene conversion events while stimulating homologous recombination in human cells in culture.
    Mol Cell Biol. 1990 Feb;10(2):785-93 PMID: 2405255
  45. A defect in mismatch repair in Saccharomyces cerevisiae stimulates ectopic recombination between homeologous genes by an excision repair dependent process.
    Genetics. 1990 Nov;126(3):535-47 PMID: 2249754
  46. A unique pathway of double-strand break repair operates in tandemly repeated genes.
    Mol Cell Biol. 1991 Mar;11(3):1222-31 PMID: 1996088
  47. Characterization of recombination intermediates from DNA injected into Xenopus laevis oocytes: evidence for a nonconservative mechanism of homologous recombination.
    Mol Cell Biol. 1991 Jun;11(6):3278-87 PMID: 2038331
Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.6.4173-4182.1994
Published
1994-06-00
Pages
4173-82
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358783
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.14.6.4173-4182.1994 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: product@genelibs.com

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2