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PMID: 8196653 Published · ppublish English Comparative Study Journal Article

Functional substitution of an essential yeast RNA polymerase subunit by a highly conserved mammalian counterpart.

Molecular and cellular biology ·Vol. 14 ·No. 6 ·1994-06-00 ·Pages 4155-9

McKune K, Woychik NA

Abstract

We isolated the cDNA encoding the homolog of the Saccharomyces cerevisiae nuclear RNA polymerase common subunit RPB6 from hamster CHO cells. Alignment of yeast RPB6 with its mammalian counterpart revealed that the subunits have nearly identical carboxy-terminal halves and a short acidic region at the amino terminus. Remarkably, the length and amino acid sequence of the hamster RPB6 are identical to those of the human RPB6 subunit. The conservation in sequence from lower to higher eukaryotes also reflects conservation of function in vivo, since hamster RPB6 supports normal wild-type yeast cell growth in the absence of the essential gene encoding RPB6.

MeSH Terms
Amino Acid Sequence Animals Base Sequence CHO Cells Cell Nucleus/enzymology Conserved Sequence Cricetinae DNA, Complementary/isolation & purification,metabolism DNA-Directed RNA Polymerases/biosynthesis,chemistry,isolation & purification Humans Macromolecular Substances Mammals Molecular Sequence Data RNA Polymerase II/biosynthesis,chemistry,isolation & purification Recombinant Fusion Proteins/biosynthesis,chemistry,isolation & purification Restriction Mapping Saccharomyces cerevisiae/enzymology Sequence Homology, Amino Acid
Chemicals
DNA, Complementary Macromolecular Substances Recombinant Fusion Proteins RNA Polymerase II DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McKune K
Roche Institute of Molecular Biology, Nutley, New Jersey 07110.
Woychik N A
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36 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.14.6.4155-4159.1994
Published
1994-06-00
Pages
4155-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC358781
Subset
IM
Databases
GENBANK
S69934
External Links
PubMed source DOI: 10.1128/mcb.14.6.4155-4159.1994 PMC Full Text View journal details
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