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PMID: 8194527 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

PIK1, an essential phosphatidylinositol 4-kinase associated with the yeast nucleus.

The EMBO journal ·Vol. 13 ·No. 10 ·1994-05-15 ·Pages 2352-61

Garcia-Bustos JF, Marini F, Stevenson I, Frei C, Hall MN

Abstract

Transmission of mitogenic and developmental signals to intracellular targets is often mediated by inositol derivatives. Here we present the cloning and characterization of a gene from Saccharomyces cerevisiae, PIK1, encoding the enzyme that catalyses the first committed step in the production of the second messenger inositol-1,4,5-trisphosphate. PIK1 encodes a phosphatidylinositol 4-kinase (PI 4-kinase) essential for growth. Cells carrying PIK1 on a multicopy vector overexpress PI 4-kinase activity exclusively in a nuclear fraction, suggesting that PIK1 is part of a nuclear phosphoinositide cycle. Temperature-sensitive mutations, but not a null mutation, can be suppressed by high osmolarity or an elevated concentration of Ca2+. Conditional mutants have a cytokinesis defect as indicated by a uniform terminal phenotype of cells with large buds and fully divided nuclei. We suggest that PIK1 controls cytokinesis through the actin cytoskeleton.

Related Genes
PIK1
MeSH Terms
1-Phosphatidylinositol 4-Kinase Amino Acid Sequence Base Sequence Cell Compartmentation Cell Division/genetics Cell Nucleus/chemistry Cloning, Molecular Genes, Fungal/genetics Genetic Complementation Test Inositol 1,4,5-Trisphosphate/biosynthesis Molecular Sequence Data Phosphotransferases (Alcohol Group Acceptor)/biosynthesis,genetics Promoter Regions, Genetic/genetics Recombinant Proteins/biosynthesis Saccharomyces cerevisiae/genetics Saccharomyces cerevisiae Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid
Chemicals
Recombinant Proteins Saccharomyces cerevisiae Proteins Inositol 1,4,5-Trisphosphate Phosphotransferases (Alcohol Group Acceptor) 1-Phosphatidylinositol 4-Kinase PIK1 protein, S cerevisiae
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Garcia-Bustos J F
Department of Biochemistry, University of Basel, Switzerland.
Marini F
Stevenson I
Frei C
Hall M N
References (67)
67 references, click to expand
  1. Calcium mobilization is required for nuclear vesicle fusion in vitro: implications for membrane traffic and IP3 receptor function.
    Cell. 1993 Jul 2;73(7):1411-22 PMID: 8391933
  2. Platelet-derived growth factor-induced phosphatidylinositol 3-kinase activation mediates actin rearrangements in fibroblasts.
    Biochem J. 1994 Mar 15;298 Pt 3:517-20 PMID: 8141762
  3. Purification and characterization of a soluble phosphatidylinositol 4-kinase from the yeast Saccharomyces cerevisiae.
    J Biol Chem. 1992 Nov 25;267(33):24117-25 PMID: 1331109
  4. Cloning and sequencing of the Saccharomyces cerevisiae gene LYP1 coding for a lysine-specific permease.
    Yeast. 1993 Jul;9(7):771-82 PMID: 8368011
  5. The biochemistry of 3'-end cleavage and polyadenylation of messenger RNA precursors.
    Annu Rev Biochem. 1992;61:419-40 PMID: 1353951
  6. Target of rapamycin in yeast, TOR2, is an essential phosphatidylinositol kinase homolog required for G1 progression.
    Cell. 1993 May 7;73(3):585-96 PMID: 8387896
  7. Genetic and biochemical characterization of a phosphatidylinositol-specific phospholipase C in Saccharomyces cerevisiae.
    Mol Cell Biol. 1993 Sep;13(9):5861-76 PMID: 8395015
  8. The molecular heterogeneity of protein kinase C and its implications for cellular regulation.
    Nature. 1988 Aug 25;334(6184):661-5 PMID: 3045562
  9. myo-inositol metabolites as cellular signals.
    Eur J Biochem. 1990 Oct 5;193(1):1-18 PMID: 2171926
  10. A role for the transcription factors Mbp1 and Swi4 in progression from G1 to S phase.
    Science. 1993 Sep 17;261(5128):1551-7 PMID: 8372350
  11. The polyphosphoinositide cycle exists in the nuclei of Swiss 3T3 cells under the control of a receptor (for IGF-I) in the plasma membrane, and stimulation of the cycle increases nuclear diacylglycerol and apparently induces translocation of protein kinase C to the nucleus.
    EMBO J. 1991 Nov;10(11):3207-14 PMID: 1655412
  12. The putative phosphoinositide-specific phospholipase C gene, PLC1, of the yeast Saccharomyces cerevisiae is important for cell growth.
    Proc Natl Acad Sci U S A. 1993 Mar 1;90(5):1804-8 PMID: 8383328
  13. A Ca(2+)-independent protein kinase C from fission yeast.
    J Biol Chem. 1993 Apr 5;268(10):7401-6 PMID: 8463273
  14. Phosphatidylinositol 4-kinase from Saccharomyces cerevisiae. Kinetic analysis using Triton X-100/phosphatidylinositol-mixed micelles.
    J Biol Chem. 1991 Jul 25;266(21):13859-65 PMID: 1649831
  15. In vitro mutagenesis and plasmid shuffling: from cloned gene to mutant yeast.
    Methods Enzymol. 1991;194:302-18 PMID: 2005795
  16. Purification, characterization, and immunofluorescence localization of Saccharomyces cerevisiae capping protein.
    J Cell Biol. 1992 Jun;117(5):1067-76 PMID: 1315784
  17. Oncogenes and signal transduction.
    Cell. 1991 Jan 25;64(2):281-302 PMID: 1846320
  18. A differential location of phosphoinositide kinases, diacylglycerol kinase, and phospholipase C in the nuclear matrix.
    J Biol Chem. 1992 Mar 15;267(8):5078-84 PMID: 1312084
  19. Phosphatidylinositol 3-kinase: structure and expression of the 110 kd catalytic subunit.
    Cell. 1992 Aug 7;70(3):419-29 PMID: 1322797
  20. Phospholipase C-gamma 1 and phosphatidylinositol 3 kinase are the downstream mediators of the PDGF receptor's mitogenic signal.
    Cell. 1993 Apr 23;73(2):321-34 PMID: 7682895
  21. Activation of a low specific activity form of DNA polymerase alpha by inositol-1,4-bisphosphate.
    Cell. 1988 Aug 26;54(5):651-8 PMID: 2842061
  22. Wortmannin is a potent phosphatidylinositol 3-kinase inhibitor: the role of phosphatidylinositol 3,4,5-trisphosphate in neutrophil responses.
    Biochem J. 1993 Dec 1;296 ( Pt 2):297-301 PMID: 8257416
  23. Inositides and the nucleus and inositides in the nucleus.
    Cell. 1993 Aug 13;74(3):405-7 PMID: 8394217
  24. Nucleolar and nuclear envelope proteins of the yeast Saccharomyces cerevisiae.
    Eur J Cell Biol. 1988 Aug;46(3):554-63 PMID: 3053175
  25. Point mutations identify the conserved, intron-contained TACTAAC box as an essential splicing signal sequence in yeast.
    Cell. 1984 Mar;36(3):645-53 PMID: 6365330
  26. Each of three "TATA elements" specifies a subset of the transcription initiation sites at the CYC-1 promoter of Saccharomyces cerevisiae.
    Proc Natl Acad Sci U S A. 1985 Dec;82(24):8562-6 PMID: 3001709
  27. The intron-containing gene for yeast profilin (PFY) encodes a vital function.
    Mol Cell Biol. 1988 Dec;8(12):5108-15 PMID: 3072473
  28. Phosphorylation of rat liver nuclear envelopes. II. Characterization of in vitro lipid phosphorylation.
    J Biol Chem. 1983 Aug 10;258(15):9368-73 PMID: 6308005
  29. New yeast actin-like gene required late in the cell cycle.
    Nature. 1992 Jan 9;355(6356):179-82 PMID: 1729653
  30. Neomycin inhibits platelet functions and inositol phospholipid metabolism upon stimulation with thrombin, but not with ionomycin or 12-O-tetradecanoyl-phorbol 13-acetate.
    Eur J Biochem. 1988 Oct 15;177(1):219-23 PMID: 2846297
  31. The ADE2 gene from Saccharomyces cerevisiae: sequence and new vectors.
    Gene. 1990 Oct 30;95(1):91-8 PMID: 2253890
  32. Specific interaction between phosphatidylinositol 4,5-bisphosphate and profilactin.
    Nature. 1985 Apr 4-10;314(6010):472-4 PMID: 2984579
  33. Membrane-associated phosphatidylinositol kinase from Saccharomyces cerevisiae.
    J Bacteriol. 1983 Oct;156(1):421-3 PMID: 6311802
  34. Phosphatidylinositol 3-kinase encoded by yeast VPS34 gene essential for protein sorting.
    Science. 1993 Apr 2;260(5104):88-91 PMID: 8385367
  35. Association of phosphatidylinositol kinase activity with polyoma middle-T competent for transformation.
    Nature. 1985 May 16-22;315(6016):239-42 PMID: 2987699
  36. Phosphoinositide metabolism and the control of cell proliferation.
    Biochim Biophys Acta. 1989 Feb;948(3):327-44 PMID: 2537660
  37. Analysis of inositol metabolites produced by Saccharomyces cerevisiae in response to glucose stimulation.
    J Biol Chem. 1993 Feb 15;268(5):3374-83 PMID: 8429013
  38. Bovine brain contains two types of phosphatidylinositol kinase.
    Biochemistry. 1987 Oct 20;26(21):6845-52 PMID: 2827737
  39. Purification, characterization, and kinetic analysis of a 55-kDa form of phosphatidylinositol 4-kinase from Saccharomyces cerevisiae.
    J Biol Chem. 1992 Aug 15;267(23):16297-304 PMID: 1322897
  40. Mutants in the S. cerevisiae PKC1 gene display a cell cycle-specific osmotic stability defect.
    J Cell Biol. 1992 Mar;116(5):1221-9 PMID: 1740473
  41. Inositol 1,4,5-trisphosphate receptor localized to endoplasmic reticulum in cerebellar Purkinje neurons.
    Nature. 1989 Jun 8;339(6224):468-70 PMID: 2542801
  42. Basic local alignment search tool.
    J Mol Biol. 1990 Oct 5;215(3):403-10 PMID: 2231712
  43. mRNA cap-binding protein: cloning of the gene encoding protein synthesis initiation factor eIF-4E from Saccharomyces cerevisiae.
    Mol Cell Biol. 1987 Mar;7(3):998-1003 PMID: 3550438
  44. OSMOTIC-REMEDIAL MUTANTS. A NEW CLASSIFICATION FOR NUTRITIONAL MUTANTS IN YEAST.
    Genetics. 1964 Nov;50:829-39 PMID: 14239771
  45. Properties of a 19-kDa Zn2+-binding protein and sequence of the Zn2+-binding domains.
    Eur J Biochem. 1988 Nov 15;177(3):561-8 PMID: 3197718
  46. Cell cycle control of the yeast HO gene: cis- and trans-acting regulators.
    Cell. 1987 Feb 13;48(3):389-97 PMID: 3542227
  47. The yeast PHO5 promoter: phosphate-control elements and sequences mediating mRNA start-site selection.
    Proc Natl Acad Sci U S A. 1987 Mar;84(5):1340-4 PMID: 2881299
  48. A mutation in PLC1, a candidate phosphoinositide-specific phospholipase C gene from Saccharomyces cerevisiae, causes aberrant mitotic chromosome segregation.
    Mol Cell Biol. 1993 Jul;13(7):4351-64 PMID: 8391635
  49. Phosphatidylinositol 4-kinase: gene structure and requirement for yeast cell viability.
    Science. 1993 Nov 26;262(5138):1444-8 PMID: 8248783
  50. Phosphoinositide kinases.
    Biochemistry. 1990 Dec 25;29(51):11147-56 PMID: 2176895
  51. Nuclear protein localization.
    Biochim Biophys Acta. 1991 Mar 7;1071(1):83-101 PMID: 2004116
  52. Yeast cell-free nuclear protein import requires ATP hydrolysis.
    Exp Cell Res. 1991 Jan;192(1):213-9 PMID: 1984412
  53. Inositol phosphates and cell signalling.
    Nature. 1989 Sep 21;341(6239):197-205 PMID: 2550825
  54. The osmotic integrity of the yeast cell requires a functional PKC1 gene product.
    Mol Cell Biol. 1992 Nov;12(11):4896-905 PMID: 1406668
  55. Phosphatidylinositol 3-kinase and its novel product, phosphatidylinositol 3-phosphate, are present in Saccharomyces cerevisiae.
    J Biol Chem. 1989 Dec 5;264(34):20181-4 PMID: 2555343
  56. Formation of phosphatidylinositol 3-phosphate by isomerization from phosphatidylinositol 4-phosphate.
    Proc Natl Acad Sci U S A. 1991 Oct 15;88(20):9184-7 PMID: 1656463
  57. The nuclear pore complex.
    J Cell Biol. 1993 Sep;122(5):977-84 PMID: 8354697
  58. An essential role for a protein and lipid kinase complex in secretory protein sorting.
    Trends Cell Biol. 1992 Dec;2(12):363-8 PMID: 14731957
  59. NSP1: a yeast nuclear envelope protein localized at the nuclear pores exerts its essential function by its carboxy-terminal domain.
    Cell. 1990 Jun 15;61(6):979-89 PMID: 2112428
  60. Transformation by ras oncogene induces nuclear shift of protein kinase C.
    Biochem Biophys Res Commun. 1990 Dec 14;173(2):528-33 PMID: 2260965
  61. Determinants for glycophospholipid anchoring of the Saccharomyces cerevisiae GAS1 protein to the plasma membrane.
    Mol Cell Biol. 1991 Jan;11(1):27-37 PMID: 1824714
  62. A yeast operator overlaps an upstream activation site.
    Cell. 1987 Jul 31;50(3):369-77 PMID: 3301002
  63. Toward a more complete 3-D structure of the nuclear pore complex.
    J Struct Biol. 1991 Dec;107(3):291-308 PMID: 1725493
  64. New yeast-Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites.
    Gene. 1988 Dec 30;74(2):527-34 PMID: 3073106
  65. Is there a relationship between phosphatidylinositol trisphosphate and F-actin polymerization in human neutrophils?
    J Biol Chem. 1990 Oct 5;265(28):16725-8 PMID: 2211588
  66. Phospholipids in the nucleus--metabolism and possible functions.
    Semin Cell Biol. 1992 Aug;3(4):225-35 PMID: 1330068
  67. TOR1 and TOR2 are structurally and functionally similar but not identical phosphatidylinositol kinase homologues in yeast.
    Mol Biol Cell. 1994 Jan;5(1):105-18 PMID: 8186460
Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1994-05-15
Pages
2352-61
Language
English
Region
England
NLM ID
8208664
PMCID
PMC395100
Subset
IM
Databases
GENBANK
X76058
External Links
PubMed source PMC Full Text View journal details
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