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PMID: 818999 Published · ppublish English Journal Article

Inhibition of leucyl-transfer ribonucleic acid synthetasymol.

The Biochemical journal ·Vol. 152 ·No. 3 ·1975-12-00 ·Pages 511-5

Ogilvie A, Wiebauer K, Kersten W

Abstract

The bacteriostatic effect of low concentrations of the antibiotic granaticin on Bacillus subtilis is relieved by the addition leucine to the growth medium. In cells treated with granaticin, aminoacylation of leucine tRNA is specifically decreased, but the content of free leucine is not. It is concluded that granaticin interferes with the charging process of leucine tRNA in B. subtilis leading to leucine auxotrophy.

MeSH Terms
Amino Acids/analysis Amino Acyl-tRNA Synthetases/antagonists & inhibitors Anti-Bacterial Agents/pharmacology Bacillus subtilis/drug effects Bacterial Proteins/biosynthesis DNA/biosynthesis Leucine/pharmacology Leucine-tRNA Ligase/antagonists & inhibitors Naphthoquinones Quinones/pharmacology RNA/biosynthesis Time Factors
Chemicals
Amino Acids Anti-Bacterial Agents Bacterial Proteins Naphthoquinones Quinones RNA DNA granaticin Amino Acyl-tRNA Synthetases Leucine-tRNA Ligase Leucine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Ogilvie A
Wiebauer K
Kersten W
References (15)
15 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1520511
Published
1975-12-00
Pages
511-5
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1172503
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1520511 PMC Full Text View journal details
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